The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/50 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
Expressed in B cell lines and T lymphocytes.
Belongs to the selectin/LECAM family. Contains 1 C-type lectin domain. Contains 1 EGF-like domain. Contains 2 Sushi (CCP/SCR) domains.