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Our Abpromise guarantee covers the use of ab52068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10µl for 106 cells. in 100 µl PBS, or for 100µl of blood.
ab18455-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|Functional Studies||Use at an assay dependent concentration. Can be used for monitoring of T cells in peripheral blood; study of Cell-adhesion molecules; study of B cell functions; study of lymphomas and leukemias.|
ab52068 staining human granulocytes in whole blood by Flow Cytometery. Cells were treated with Optilyse C (containing 1.5 % paraformaldehyde) solution and gated by electronic volume and side scatter. The primary antibody was diluted 1/10 and incubated with sample for 30 minutes at 21°C.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"