製品の概要

  • 製品名Anti-CD62E antibody
    CD62E 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CD62E
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide surrounding aa 584 of human CD62E

  • ポジティブ・コントロール
    • Jurkat cell lysate, mouse small intestine and rat kidney tissue lysate.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 67 kDa.Can be blocked with CD62E peptide (ab113900).

ターゲット情報

  • 機能Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
  • 配列類似性Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 6 Sushi (CCP/SCR) domains.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD26E antibody
    • CD62 antigen-like family member E antibody
    • CD62E antibody
    • E selectin antibody
    • E-selectin antibody
    • ELAM antibody
    • ELAM-1 antibody
    • ELAM1 antibody
    • Endothelial adhesion molecule 1 antibody
    • Endothelial leukocyte adhesion molecule 1 antibody
    • ESEL antibody
    • LECAM2 antibody
    • Leukocyte-endothelial cell adhesion molecule 2 antibody
    • LYAM2_HUMAN antibody
    • Sele antibody
    • Selectin E antibody
    see all

Anti-CD62E antibody 画像

  • ab18981 staining CD62E in Rat brain microvascular endothelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/50 in 1% BSA) for 18 hours at 4°C. A donkey anti-rabbit DyLight® 650 (IgG; 1/500) was used as the secondary antibody.

    See Abreview

  • Anti-CD62E antibody (ab18981) at 0.5 µg/ml + Jurkat

    Predicted band size : 67 kDa

    Secondary antibody - goat anti-rabbit HRP (ab6721)

  • Anti-CD62E antibody (ab18981) at 2 µg/ml + SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 67 kDa
    Observed band size : 67 kDa

Anti-CD62E antibody (ab18981) 使用論文

This product has been referenced in:
  • Wu F  et al. CXCR2 is essential for cerebral endothelial activation and leukocyte recruitment during neuroinflammation. J Neuroinflammation 12:98 (2015). WB ; Mouse . Read more (PubMed: 25990934) »
  • Kirui DK  et al. Transient mild hyperthermia induces E-selectin mediated localization of mesoporous silicon vectors in solid tumors. PLoS One 9:e86489 (2014). WB ; Human . Read more (PubMed: 24558362) »

See all 8 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (293-T)
Permeabilization Yes - .1% triton
Specification 293-T
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde
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投稿 Nov 30 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample Rat Cell (Rat Brain Microvascular Endothelial Cells (RBMVEC))
Specification Rat Brain Microvascular Endothelial Cells (RBMVEC)
Permeabilization No
Fixative Paraformaldehyde
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投稿 Nov 19 2013

1. Deparaffinize/hydrate sections:
a) Incubate sections in three washes of xylene for 5 minutes each.
b) Incubate sections in two washes of 100% ethanol for 10 minutes each.
c) Incubate sections in two washes of 95% ethanol for 10 minute...

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Thank you for your enquiry.

I can confirm that ab18414 can be stored at 4oC as described on the datasheet. This should not be aliquotted and frozen.

The others I would recommend to aliquot and freeze immediately. Keeping them at 4...

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I am very pleased to hear you would like to accept our offer and test ab54426 in IHC-P.

DISCOUNT CODE: ######

Expiration date: ######

This code will give you: 1 free primary antibody before the expiration date. To redee...

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Thank you for contacting us.
I am sorry to hear that the antibody does not seem to work as expected.

As we had discussed, I would suggest to try the following tips to reduce the background as well as shift the MW to a higher band:
<...

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I agree with Heather that it would be useful to have a negative control sample. I may be mistaken but if E-selectin is only expressed exclusively in endothelial cells, perhaps using an extract from a different cell type as a negative control ...

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Thank you for your phone call. I just wanted to follow up with you and send you the suggestions we had discussed over the phone: 1) use peptide in 10-fold excess 2) incubate peptide with Ab for 1-2 h at RT before using for WB 3) avoid prepa...

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