Anti-CD59 抗体 [p282 (H19)] (ab79520)


  • 製品名
    Anti-CD59 antibody [p282 (H19)]
    CD59 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [p282 (H19)] to CD59
  • 由来種
  • アプリケーション
    適用あり: IHC-P, Flow Cyt, WBmore details
  • 種交差性
    交差種: Human, Chimpanzee, Monkey, Baboon
  • 免疫原

    Full length protein corresponding to human CD59

  • ポジティブ・コントロール
    • Placenta tissue



Our Abpromise guarantee covers the use of ab79520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 2µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.


  • 機能
    Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
    The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
  • 関連疾患
    Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300].
  • 配列類似性
    Contains 1 UPAR/Ly6 domain.
  • 翻訳後修飾
    N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
    Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.
  • 細胞内局在
    Cell membrane. Secreted. Soluble form found in a number of tissues.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 16.3A5 antibody
    • 1F5 antibody
    • 1F5 antigen antibody
    • 20 kDa homologous restriction factor antibody
    • CD 59 antibody
    • CD_antigen=CD59 antibody
    • CD59 antibody
    • CD59 antigen antibody
    • CD59 antigen complement regulatory protein antibody
    • CD59 antigen p18 20 antibody
    • CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) antibody
    • CD59 glycoprotein antibody
    • CD59 molecule antibody
    • CD59 molecule complement regulatory protein antibody
    • CD59_HUMAN antibody
    • Cd59a antibody
    • Complement regulatory protein antibody
    • EJ16 antibody
    • EJ30 antibody
    • EL32 antibody
    • FLJ38134 antibody
    • FLJ92039 antibody
    • G344 antibody
    • HRF 20 antibody
    • HRF-20 antibody
    • HRF20 antibody
    • Human leukocyte antigen MIC11 antibody
    • Ly 6 like protein antibody
    • Lymphocytic antigen CD59/MEM43 antibody
    • MAC inhibitory protein antibody
    • MAC IP antibody
    • MAC-inhibitory protein antibody
    • MAC-IP antibody
    • MACIF antibody
    • MACIP antibody
    • MEM43 antibody
    • MEM43 antigen antibody
    • Membrane attack complex (MAC) inhibition factor antibody
    • Membrane attack complex inhibition factor antibody
    • Membrane inhibitor of reactive lysis antibody
    • MGC2354 antibody
    • MIC11 antibody
    • MIN1 antibody
    • MIN2 antibody
    • MIN3 antibody
    • MIRL antibody
    • MSK21 antibody
    • p18 20 antibody
    • Protectin antibody
    • Surface antigen recognized by monoclonal antibody 16.3A5 antibody
    • T cell activating protein antibody
    see all


  • ab79520 at 10µg/ml staining CD59 by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded placenta sections).
  • Overlay histogram showing Jurkat cells stained with ab79520 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79520, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Anti-CD59 antibody [p282 (H19)] (ab79520) at 1/2000 dilution + E. coli expressed mature human CD59 refolded at 0.5 µg

    HRP-conjugated rabbit anti-mouse IgG polyclonal at 1/2000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 14 kDa
    Observed band size: 9 kDa (why is the actual band size different from the predicted?)

    Exposure time: 20 seconds

    See Abreview


ab79520 has not yet been referenced specifically in any publications.

レビューと Q&A

Rhesus monkey Cultured Cells (RF/6A chorioretinal endothelial cells)
RF/6A chorioretinal endothelial cells

Andrea Cabrera

Verified customer

投稿 Sep 12 2016

Western blot
Loading amount
0.5 µg
Gel Running Conditions
Non-reduced Denaturing (12% polyacrylamide gel run in Novex bolt MES SDS running buffer)
Human Purified protein (E. coli expressed mature human CD59 refolded and p)
E. coli expressed mature human CD59 refolded and p
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3µg/mL · Temperature: 21°C


Verified customer

投稿 Aug 14 2014