製品の概要

  • 製品名Anti-CD59 antibody [MEM-43/5]
    CD59 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-43/5] to CD59
  • 特異性CD59 antigen (human). MEM-43/5 reacts with well defined epitope (around L33) and does not compete with MEM-43 and many other CD59 antibodies
  • アプリケーション適用あり: ICC/IF, IHC-P, WB, IP, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Thymocytes and T lymphocytes.

  • エピトープThe antibody MEM-43/5 reacts with well defined epitope around L33 (see Bodian et al)
  • ポジティブ・コントロール
    • Flow cyt: blood Jeg3 cell line IF/ICC

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. CD59 is GPI-anchored, so we recommend to use a laurylmatoside based lysis buffer or triton base buffer (see Bodian et al; 1% Triton X-100, 1 µg/ml leupeptin, 1 µg/ml pepstatin A and 1 mM phenlymethylsulphonyl fluoride in PBS), not NP40.
IP Use at an assay dependent concentration.
Flow Cyt Use a concentration of 1 µg/ml. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
    The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
  • 関連疾患Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300].
  • 配列類似性Contains 1 UPAR/Ly6 domain.
  • 翻訳後修飾N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
    Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.
  • 細胞内局在Cell membrane. Secreted. Soluble form found in a number of tissues.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 16.3A5 antibody
    • 1F5 antibody
    • 1F5 antigen antibody
    • 20 kDa homologous restriction factor antibody
    • CD 59 antibody
    • CD_antigen=CD59 antibody
    • CD59 antibody
    • CD59 antigen antibody
    • CD59 antigen complement regulatory protein antibody
    • CD59 antigen p18 20 antibody
    • CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) antibody
    • CD59 glycoprotein antibody
    • CD59 molecule antibody
    • CD59 molecule complement regulatory protein antibody
    • CD59_HUMAN antibody
    • Cd59a antibody
    • Complement regulatory protein antibody
    • EJ16 antibody
    • EJ30 antibody
    • EL32 antibody
    • FLJ38134 antibody
    • FLJ92039 antibody
    • G344 antibody
    • HRF 20 antibody
    • HRF-20 antibody
    • HRF20 antibody
    • Human leukocyte antigen MIC11 antibody
    • Ly 6 like protein antibody
    • Lymphocytic antigen CD59/MEM43 antibody
    • MAC inhibitory protein antibody
    • MAC IP antibody
    • MAC-inhibitory protein antibody
    • MAC-IP antibody
    • MACIF antibody
    • MACIP antibody
    • MEM43 antibody
    • MEM43 antigen antibody
    • Membrane attack complex (MAC) inhibition factor antibody
    • Membrane attack complex inhibition factor antibody
    • Membrane inhibitor of reactive lysis antibody
    • MGC2354 antibody
    • MIC11 antibody
    • MIN1 antibody
    • MIN2 antibody
    • MIN3 antibody
    • MIRL antibody
    • MSK21 antibody
    • p18 20 antibody
    • Protectin antibody
    • Surface antigen recognized by monoclonal antibody 16.3A5 antibody
    • T cell activating protein antibody
    see all

Anti-CD59 antibody [MEM-43/5] 画像

  • IHC image of ab9183 staining CD59 in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab9183, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ICC/IF image of ab9183 stained Jeg3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9183, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Overlay histogram showing Jurkat cells stained with ab9183 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9183, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-CD59 antibody [MEM-43/5] (ab9183) 使用論文

This product has been referenced in:
  • Cai H  et al. Differential transformation capacity of Src family kinases during the initiation of prostate cancer. Proc Natl Acad Sci U S A 108:6579-84 (2011). WB ; Human . Read more (PubMed: 21464326) »
  • Renner B  et al. The complement inhibitors Crry and factor H are critical for preventing autologous complement activation on renal tubular epithelial cells. J Immunol 185:3086-94 (2010). Flow Cyt . Read more (PubMed: 20675597) »

See all 5 Publications for this product

Product Wall

Thank you very much for your interest in ab9183 and ab30532.
As discussed over the phone yesterday, to our knowledge, ab9183 has not been tested in IHC frozen sections. This does not mean that it will not work, simply that we have not tested it ou...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (placenta)
Specification placenta
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
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Verified customer

投稿 Apr 09 2012

Thank you for calling Abcam.

I am sorry that you were having problems with ab9182 in IHC-P. As we discussed, I am sending you ab9183 as a free of charge replacement and you should have it by 10.30am tomorrow morning. You r new order number is...

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Thank you for your patience. I am sorry to hear that you have been experiencing problems with ab9183 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that ...

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Thank you for taking the time to contact us. I am sorry to hear the customer has difficulty obtaining satisfactory results from this antibody (ab9183). CD59E is a glycolipid-anchored protein, and these anchored membrane proteins tend to form deterg...

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Thank you for your enquiry. Here is the answer from the originator: "CD59 is small glycoprotein, is SDS-PAGE it migrates about 18-25 KDa due to carbohydrate moiety (if treated with endoglycosidases, it moves faster). It is GPI-anchored, so that th...

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The blocking potential of this antibody has not yet been determined, nor has it's cross-reactivity with porcine CD59.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"