The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報Flow Cyt: 1/50 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
IHC-Fr: 1/10 - 1/100.
IHC-P: 1/10 - 1/50.
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but sodium citrate buffer may enhance staining.
Is unsuitable for IP or WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
組織特異性Mainly expressed in the brain.
パスウェイProtein modification; protein glycosylation.
配列類似性Belongs to the glycosyltransferase 43 family.