製品の概要

  • 製品名
    Anti-CD55 antibody [143-30] (FITC)
    CD55 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [143-30] to CD55 (FITC)
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • 特異性
    ab25634 recognises CD55, also known as decay accelerating factor (DAF).
  • アプリケーション
    適用あり: Flow Cyt, IHC-Frmore details
  • 種交差性
    交差種: Human, Pig
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    モノクローナル
  • クローン名
    143-30
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab25634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 5µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
  • 組織特異性
    Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
  • 配列類似性
    Belongs to the receptors of complement activation (RCA) family.
    Contains 4 Sushi (CCP/SCR) domains.
  • ドメイン
    The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3.
  • 翻訳後修飾
    The Ser/Thr-rich domain is heavily O-glycosylated.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 55 antibody
    • CD55 antibody
    • CD55 antigen antibody
    • CD55 Cromer blood group system antibody
    • CD55 molecule (Cromer blood group) antibody
    • CD55 molecule antibody
    • CD55 molecule, decay accelerating factor for complement (Cromer blood group) antibody
    • Cd55a antibody
    • Complement decay accelerating factor antibody
    • Complement decay-accelerating factor antibody
    • Complement decay-accelerating factor, GPI-anchored antibody
    • CR antibody
    • CROM antibody
    • Cromer Blood Group antigen antibody
    • Cromer blood group system antibody
    • DAF antibody
    • Daf-GPI antibody
    • DAF_HUMAN antibody
    • Daf1 antibody
    • Dcay accelerating factor for complement (CD55, Cromer blood group system) antibody
    • Decay accelarating factor 1, isoform CRA_a antibody
    • Decay accelerating factor (GPI-form) antibody
    • Decay Accelerating Factor for Complement antibody
    • Decay accelerating factor GPI-form antibody
    • Decay accelerating factor soluble-form antibody
    • GPI-DAF antibody
    • TC antibody
    see all

画像

  • Overlay histogram showing peripheral blood lymphocytes stained with ab25634 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25634, 0.5 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.

参考文献

This product has been referenced in:
  • Hofmann A  et al. Proteomic cell surface phenotyping of differentiating acute myeloid leukemia cells. Blood 116:e26-34 (2010). Flow Cyt ; Human . Read more (PubMed: 20570859) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Pig Cell (fetal fibroblast transfected human CD55 gene)
Specification
fetal fibroblast transfected human CD55 gene
Preparation
Cell harvesting/tissue preparation method: Trypsin/EDTA
Sample buffer: PBS
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
CD55 expressing cells on the cell surface
Username

Abcam user community

Verified customer

投稿 May 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Blood cells)
Specification
Blood cells
Preparation
Cell harvesting/tissue preparation method: Trypsin
Sample buffer: PBS
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
10000
Username

Ms. Kim Soohyun

Verified customer

投稿 Apr 27 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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