製品の概要

  • 製品名Anti-CD53 antibody [MEM-53]
    CD53 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-53] to CD53
  • 特異性Human CD53 antigen. This antibody also recognizes the deglycosylated antigen (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
  • アプリケーション適用あり: Flow Cyt, IHC-Fr, IP, WB, ICC/IF, Functional Studiesmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Tissue/ cell preparation (Human). (Leucocytes from a patient suffering from a LGL-type leukaemia).

  • 特記事項


    The antibody reacts with CD53 antigen, a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Purified from ascites by protein A-affinity chromatography.
  • 一次抗体 備考The antibody reacts with CD53 antigen, a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.
  • ポリ/モノモノクローナル
  • クローン名MEM-53
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use a concentration of 4 µg/ml.
IHC-Fr Use at an assay dependent concentration. Can be used to discriminate lymphomas from other tumors.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 17438075
Functional Studies Use at an assay dependent concentration. Induces activation of monocytes and B lymphocytes.

ターゲット情報

  • 機能May be involved in growth regulation in hematopoietic cells.
  • 組織特異性B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.
  • 配列類似性Belongs to the tetraspanin (TM4SF) family.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI323659 antibody
    • Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
    • CD 53 antibody
    • CD53 antibody
    • CD53 antigen antibody
    • CD53 antigen, isoform CRA_b antibody
    • CD53 glycoprotein antibody
    • Cd53 molecule antibody
    • CD53 tetraspan antigen antibody
    • CD53_HUMAN antibody
    • Cell surface glycoprotein CD53 antibody
    • Leukocyte antigen MRC OX-44 antibody
    • Leukocyte surface antigen CD53 antibody
    • MOX 44 antibody
    • MOX44 antibody
    • OTTHUMP00000059505 antibody
    • Ox-44 antibody
    • OX44 antibody
    • RATOX44 antibody
    • Tetraspanin 25 antibody
    • Tetraspanin-25 antibody
    • Tetraspanin25 antibody
    • Transmembrane glycoprotein antibody
    • TSPAN 25 antibody
    • Tspan-25 antibody
    • TSPAN25 antibody
    see all

Anti-CD53 antibody [MEM-53] 画像

  • ab667 staining CD53 in human monocyte–derived macrophages by Immunocytochemistry/ Immunofluorescence. Bar 10 µm.

Anti-CD53 antibody [MEM-53] (ab667) 使用論文

This product has been referenced in:
  • Drukker M  et al. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nat Biotechnol 30:531-42 (2012). Human . Read more (PubMed: 22634564) »
  • Ruiz-Mateos E  et al. CD63 is not required for production of infectious human immunodeficiency virus type 1 in human macrophages. J Virol 82:4751-61 (2008). ICC/IF . Read more (PubMed: 18321974) »

See all 8 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"