製品の概要

  • 製品名Anti-CD52 antibody [HI186]
    CD52 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HI186] to CD52
  • 特異性CD52 antigen
  • アプリケーション適用あり: Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Tissue/ cell preparation (Human tonsil).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Purified from ascites by protein G-affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名HI186
  • ミエローマunknown
  • アイソタイプIgG2b
  • 軽鎖の種類unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use a concentration of 2 µg/ml.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能May play a role in carrying and orienting carbohydrate, as well as having a more specific role.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cambridge pathology 1 antigen antibody
    • CAMPATH 1 antibody
    • CAMPATH 1 antigen antibody
    • CAMPATH-1 antigen antibody
    • CD 52 antibody
    • CD52 antibody
    • CD52 antigen antibody
    • CD52 molecule antibody
    • CD52_HUMAN antibody
    • CDw52 antibody
    • CDW52 antigen antibody
    • Epididymal secretory protein E5 antibody
    • Epididymis secretory sperm binding protein Li 171mP antibody
    • HE 5 antibody
    • He5 antibody
    • Human epididymis-specific protein 5 antibody
    see all

Anti-CD52 antibody [HI186] 画像

  • Overlay histogram showing Jurkat cells stained with ab2576 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2576, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-CD52 antibody [HI186] (ab2576) 使用論文

This product has been referenced in:

See 1 Publication for this product

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Thank you for your e-mails and for providing further information about your protocol. We are very sorry to hear that you are having problem with this antibody. We have searched our database and found that this is a popular selling product and your ...

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It has been confirmed by HLDA Workshop VI (the best proof of defined specificity). If you have any additional questions, please contact us again.

Immunogen was a human tonsil=tissue in suspension.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"