製品の概要

  • 製品名Anti-CD45RA antibody [MEM-56]
    CD45RA 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-56] to CD45RA
  • 特異性CD45RA antigen (human)
  • アプリケーション適用あり: IHC-P, WB, Flow Cyt, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Tissue/ cell preparation (Human). (Thymocytes and T lymphocytes).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.
WB Use at an assay dependent concentration. Cross reacts with an intracellular 60 kDa protein of unknown identity like other anti-CD45RA MAbs.
Flow Cyt Use a concentration of 1 - 4 µg/ml. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
  • 関連疾患Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
    Multiple sclerosis
  • 配列類似性Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • ドメインThe first PTPase domain interacts with SKAP1.
  • 翻訳後修飾Heavily N- and O-glycosylated.
  • 細胞内局在Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B220 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • EC 3.1.3.48 antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • Loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lymphocyte antigen 5 antibody
    • Lyt-4 antibody
    • Protein tyrosine phosphatase, receptor type, C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • RT7 antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Anti-CD45RA antibody [MEM-56] 画像

  • Lane 1 : CD45 control antibody
    Lanes 2 - 3 : Anti-CD45RA antibody [MEM-56] (ab8217)

    Lane 1 : HUT-78 human cutaneous T cell lymphoma cell line.
    Lane 2 : sample of cell lysate prepared under non-reducing conditions
    Lane 3 : sample of cell lysate prepared under reducing conditions

Anti-CD45RA antibody [MEM-56] (ab8217) 使用論文

This product has been referenced in:
  • Abdulamir AS  et al. Changing survival, memory cell compartment, and T-helper balance of lymphocytes between severe and mild asthma. BMC Immunol 9:73 (2008). ICC ; Human . Read more (PubMed: 19087256) »
  • Bazil V  et al. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) 35:289-97 (1989). Read more (PubMed: 2534480) »

See all 3 Publications for this product

Product Wall

Thank you for your patience in this matter. In regards to ab30476 (CD45RA antibody [F8-11-13] (Biotin)), the originator does not know which epitope this antibody is recognizing. However, information available in the literature suggests that it reco...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"