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Our Abpromise guarantee covers the use of ab64100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.25µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
ab64100 staining CD45R in mouse lympn node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 25 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/100 in 1% BSA/TPBS) for 16 hours at 25°C. An undiluted HRP-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody.
ab64100 staining CD45R in mouse bone marrow cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 5% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/400 in PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rat IgG polyclonal (1/500) was used as the secondary antibody.