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A synthetic peptide corresponding to residues in the leukocyte common antigen domain of human CD45
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our Abpromise guarantee covers the use of ab32437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 147 kDa).|
Human whole blood was stained with anti-CD45 antibody ab32437 (red). In brief, the erythrocytes were lysed and the cells were then stained with anti-CD45 (ab32437) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black) was rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue) was also used as a control.
Acquisition of >30,000 total events were collected. Gating strategy - peripheral blood lymphocytes
ab32437 has not yet been referenced specifically in any publications.