製品の概要

  • 製品名Anti-CD45 antibody [MEM-28]
    CD45 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-28] to CD45
  • 特異性Human CD45 antigen (LCA). This antibody reacts with all alternative forms of CD45.
  • アプリケーション適用あり: Flow Cyt, IP, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Tissue, cells or virus corresponding to Human CD45. Human thymocytes and T lymphocytes.

  • ポジティブ・コントロール
    • Jurkat human leukemia T-cell lysate; Kg-1a human leukemia cell lysate, Human peripheral blood mononuclear cells

アプリケーション

Our Abpromise guarantee covers the use of ab8216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

(Recognizes an extracellular epitope).
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Antigen retrieval is not essential but may optimise staining.
WB Use a concentration of 1 µg/ml.

Prepare and run samples in non-reducing conditions.

ICC/IF Use a concentration of 10 µg/ml.

PubMed: 23908685

ターゲット情報

  • 機能Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • 関連疾患Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • 配列類似性Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • ドメインThe first PTPase domain interacts with SKAP1.
  • 翻訳後修飾Heavily N- and O-glycosylated.
  • 細胞内局在Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B220 antibody
    • CD 45 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lyt-4 antibody
    • OTTHUMP00000033813 antibody
    • OTTHUMP00000033816 antibody
    • OTTHUMP00000033817 antibody
    • OTTHUMP00000038574 antibody
    • Protein tyrosine phosphatase receptor type c polypeptide antibody
    • protein tyrosine phosphatase, receptor type, C antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Anti-CD45 antibody [MEM-28] 画像

  • Ab8216 staining human normal tonsil tissue. Staining is localised to cellular membranes.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Overlay histogram showing peripheral blood lymphocytes stained with ab8216 (red line). The cells were incubated with the antibody (ab8216, 1µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/200 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.

  • Immunocytochemistry/Immunofluorescence analysis of human peripheral blood mononuclear cells labelling CD45 (green) with ab8216 at 10 μg/mL. Nuclei were counterstained with DAPI (blue).

Anti-CD45 antibody [MEM-28] (ab8216) 使用論文

This product has been referenced in:
  • Nel I  et al. Circulating Tumor Cell Composition in Renal Cell Carcinoma. PLoS One 11:e0153018 (2016). ICC/IF ; Human . Read more (PubMed: 27101285) »
  • Lunardi S  et al. IP-10/CXCL10 induction in human pancreatic cancer stroma influences lymphocytes recruitment and correlates with poor survival. Oncotarget 5:11064-80 (2014). IHC-P ; Human . Read more (PubMed: 25415223) »

See all 18 Publications for this product

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There are 3-8 molecules of biotin at each antibody molecule.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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IF by definition refers to any antibody application that utilizes fluorescence as the detection method. Thus, technically ICC and IF are one in the same, and you could argue that IHC is also a form of IF.

For the purposes of searching for...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Pancreatic Cancer)
Specification Pancreatic Cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: CC1, a Tris/Borate/EDTA buffer pH 8
Permeabilization No
Blocking step Inhibitor CM as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 37°C
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Abcam user community

Verified customer

投稿 Dec 18 2012

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with ab8216. I have read your technical questionnaire and I have a few comments. You are using an extraction protocol that I would advise: RIPA buffer. I also...

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I am sorry to hear that you have been experiencing problems with ab8216 in Western blotting. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functio...

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We do not know the epitope or if this antibody recognizes any fragments less than the expected size of the protein. We do not have access to the original WB data. We did not perform such a study, and most likely, if we did and saw any smaller fragme...

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ab8216 has not been tested in mouse, so we have no further information on this. We have several CD45 antibodies so I suggest that you conduct a search through the Abcam Website. I also recommend that you should click on the links “Product Reviews” and ...

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This antibody has not been tested for cross reactivity with sheep. As noted on the datasheet, for immunohistochemistry, no treatment of parrafin sections neccessary. I suggest that you examine the degree of sequence homology between human and sheep CD4...

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No. Even if this antibody did recognise mouse CD45, the antibody has been raised in mouse, therefore the anti mouse secondary would non-specifically bind to the tissue leading to high background staining. At current we do not stock an antibody towards ...

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