製品の概要

  • 製品名Anti-CD45 antibody [IBL-3/16]
    CD45 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [IBL-3/16] to CD45
  • 特異性This antibody recognises mouse CD45. Enhances the MHC Class II induced cluster formation of mouse B cells.
  • アプリケーション適用あり: IP, ICC/IF, Flow Cyt, IHC-Frmore details
    適用なし: IHC-P,IHC-R or WB
  • 種交差性
    交差種: Mouse
    非交差種: Rat, Human
  • 免疫原

    Purified B cells from mouse lymph nodes.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab23910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/1000. Use 50µl of the suggested working dilution to label 105 cells in 100µl.



ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/500.
  • 追加情報Is unsuitable for IHC-P,IHC-R or WB.
  • ターゲット情報

    • 機能Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
    • 関連疾患Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
      Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
    • 配列類似性Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 tyrosine-protein phosphatase domains.
    • ドメインThe first PTPase domain interacts with SKAP1.
    • 翻訳後修飾Heavily N- and O-glycosylated.
    • 細胞内局在Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt
    • 参照データベース
    • 別名
      • B220 antibody
      • CD 45 antibody
      • CD45 antibody
      • CD45 antigen antibody
      • CD45R antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lyt-4 antibody
      • OTTHUMP00000033813 antibody
      • OTTHUMP00000033816 antibody
      • OTTHUMP00000033817 antibody
      • OTTHUMP00000038574 antibody
      • Protein tyrosine phosphatase receptor type c polypeptide antibody
      • protein tyrosine phosphatase, receptor type, C antibody
      • Ptprc antibody
      • PTPRC_HUMAN antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    Anti-CD45 antibody [IBL-3/16] 画像

    • ab23910 staining the CD45 in Mouse brain tissue sections by IHC-Fr (frozen sections). Tissue was fixed with acetone and blocked with 5% BSA for 10 hours at 20°C. Samples were incubated with primary antibody (1/100) for 1 hour at 20°C. An Alexa Fluor®-conjugated goat anti-rat IgG polyclonal (1/300) was used as the secondary antibody.

      See Abreview

    Anti-CD45 antibody [IBL-3/16] (ab23910) 使用論文

    This product has been referenced in:
    • Lee BC  et al. Deubiquitinase CYLD acts as a negative regulator for bacterium NTHi-induced inflammation by suppressing K63-linked ubiquitination of MyD88. Proc Natl Acad Sci U S A 113:E165-71 (2016). Mouse . Read more (PubMed: 26719415) »

    See 1 Publication for this product

    Product Wall

    We recommend that alignment should be over 85% to predict that an antibody will detect in a different species. The alignment between the mouse protein and the human immunogen is below the threshold at 80%. I would therefore suggest this antibody would ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (brain)
    Specification brain
    Fixative Acetone
    Permeabilization No
    Blocking step BSA as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    投稿 Apr 15 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"