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The details of the immunogen for this antibody are not available.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Our Abpromise guarantee covers the use of ab51482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|RIA||Use at an assay dependent concentration. Quantification of CD45 molecules on cell surfaces.|
|Flow Cyt||Use 0.2µg for 106 cells. ab98984-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
ab51482 staining CD45 in Mouse speen cells by Flow Cytometry. The splenocytes were dispersed with 70µm strainer and erythrolyzed in PBS. The sample was incubated with the primary antibody (undiluted) for 30 minutes at 0°C.
Gateing Strategy: FSC, SSC.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"