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The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab25386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 17579051|
|IHC-P||Use at an assay dependent concentration. PubMed: 19695260|
|Flow Cyt||Use at an assay dependent concentration.
See Haidl et al.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Flow Cytometry analysis of BALB/c mouse splenocytes labeling CD45 with ab25386 at 1 μg/106 cells (purple). A Mouse Anti-Rat IgG2b-PE was used as the secondary antibody. Grey - Isotype Control, Rat IgG2b-UNLB, followed by Mouse Anti-Rat IgG2b-PE.