製品の概要

  • 製品名Anti-CD45 antibody [Hle-1]
    CD45 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Hle-1] to CD45
  • アプリケーション適用あり: IHC-P, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Ficoll-Triosil-separated human peripheral blood mononuclear cells.

アプリケーション

Our Abpromise guarantee covers the use of ab18613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • 関連疾患Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • 配列類似性Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • ドメインThe first PTPase domain interacts with SKAP1.
  • 翻訳後修飾Heavily N- and O-glycosylated.
  • 細胞内局在Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B220 antibody
    • CD 45 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lyt-4 antibody
    • OTTHUMP00000033813 antibody
    • OTTHUMP00000033816 antibody
    • OTTHUMP00000033817 antibody
    • OTTHUMP00000038574 antibody
    • Protein tyrosine phosphatase receptor type c polypeptide antibody
    • protein tyrosine phosphatase, receptor type, C antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Anti-CD45 antibody [Hle-1] 画像

  • ab18613 staining CD45 in human synovium tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with zinc buffered formalin and blocked with 5% serum for 1 hour at 27°C; antigen retrieval was by heat mediation in Tris HCl pH 10. Samples were incubated with primary antibody (1/100) for 15 hours at 4°C. A Streptavidin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Ab18613 staining Human normal tonsil. Staining is localized to the cell membrane
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Overlay histogram showing peripheral blood lymphocytes stained with ab18613 (red line). The cells were incubated with the antibody (ab18613, 1µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/200 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.

Anti-CD45 antibody [Hle-1] (ab18613) 使用論文

This product has been referenced in:
  • Tchilian EZ  et al. A deletion in the gene encoding the CD45 antigen in a patient with SCID. J Immunol 166:1308-13 (2001). Read more (PubMed: 11145714) »
  • Bradstock KF  et al. Subpopulations of normal and leukemic human thymocytes: an analysis with the use of monoclonal antibodies. J Natl Cancer Inst 65:33-42 (1980). Read more (PubMed: 6993744) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris HCl ph10
Sample Human Tissue sections (Synovium)
Specification Synovium
Permeabilization No
Fixative zinc buffered formalin
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投稿 Aug 04 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"