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Tissue/ cell preparation (Human): Non T, non B ALL cell line (REH).
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab40763 as a replacement.
Our Abpromise guarantee covers the use of ab33533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. See Abreview.|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IP||Use at 2 µg/mg of lysate.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 147 kDa.|
ab33533 staining human blood mononuclear cells by ICC/IF. Cells were PFA fixed and blocked in 2.5% BSA for 30 minutes at 20°C prior to incubation with the primary antibody, diluted 1/100, for 18 hours at 4°C. A FITC conjugated goat anti-mouse IgG antibody was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"