製品の概要

  • 製品名Anti-CD45 antibody
    CD45 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CD45
  • アプリケーション適用あり: Flow Cyt, IHC-Fr, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to Human CD45 aa 900-1000 conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab17553, ab17553, ab17553, ab17553)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Jurkat whole cell lysate and Human spleen tissue sections. In Flow Cytometry, this antibody gave a positive signal in methnaol fixed Jurkat cells.

アプリケーション

Our Abpromise guarantee covers the use of ab10558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/150.
WB 1/500. Detects a band of approximately 155 kDa (predicted molecular weight: 147 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100.

ターゲット情報

  • 機能Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • 関連疾患Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • 配列類似性Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • ドメインThe first PTPase domain interacts with SKAP1.
  • 翻訳後修飾Heavily N- and O-glycosylated.
  • 細胞内局在Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B220 antibody
    • CD 45 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lyt-4 antibody
    • OTTHUMP00000033813 antibody
    • OTTHUMP00000033816 antibody
    • OTTHUMP00000033817 antibody
    • OTTHUMP00000038574 antibody
    • Protein tyrosine phosphatase receptor type c polypeptide antibody
    • protein tyrosine phosphatase, receptor type, C antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Anti-CD45 antibody 画像

  • ab10558 staining CD45 in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Negative control is shown in panel. Blocking was with horse serum (1/75) for 1 hour at room temperature. Samples were incubated with primary antibody (1/10) overnight at 4°C. A Biotin-conjugated Horse anti-mouse polyclonal (1/200) was used as the secondary antibody.

  • ab10558 staining CD45 in Mouse abdominal wall tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 24 hours at 4°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in milk) for 24 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • IHC image of CD45 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10558, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ab10558 staining CD45 in fetal mouse liver tissue sections 12.5 dpc by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and a permeabilization step was performed using 0.1% Tween-20. Samples were then blocked with 5% BSA for 1.5 hours at 20°C followed by incubation with the primary antibody at a 1/150 dilution, for 16 hours at 4°C. A Goat anti-rabbit Alexa Fluor® 594 polyclonal was used as the secondary antibody at a 1/1500 diution.

    See Abreview

  • All lanes : Anti-CD45 antibody (ab10558) at 1/500 dilution

    Lane 1 : Jurkat Whole Cell Lysate
    Lane 2 : Jurkat Whole Cell Lysate with Human CD45 peptide (ab17553)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Predicted band size : 147 kDa


    Exposure time : 3 minutes
  • Asynchronous KM-H2 cells were pelleted and labeled by indirect immunofluorescence. Cells were stained with ab10558 (1/200) for 30min at 4'C, washed and then stained with goat anti-rabbit alexafluor 488 (1/200). Forward/Side scatter were used to eliminate cellular debris. The accompanying marker was applied such that only 2% of the IgG control was positive Based on the accompanying image, approximately 8.4% of cells exhibited positive staining for anti-CD45. Since KM-H2 are known to have low levels of CD45 transcripts they are expected to have low levels of CD45, which is reflected in the ~8%. This image is from an Abreview.

  • ab10558 (1:40) staining CD45 in paraffin-embedded human tonsil (left panel) using an automated system (Ventana Discovery). Right-hand panel shows negative control (no primary antibody).
    Using this protocol there is strong membrane staining of B cells in the germinal centres and mantle zone of the follicles and scattered cells of the interfollicular areas (paracortical T and B cells). There is a mild to moderate degree of cytoplasmic staining associated with the membrane staining in these specific cells.

    Sections were rehydrated and antigen retrieved in CC1 Cell Conditioning Buffer using Ventana Extended Retrieval programme. Slides were blocked in 3% H2O2 /4 min/ 37°C and incubated with ab10558 (1:40 dilution / 1 hour/ 37°C). Sections then blocked (4mins/ 37°C) and incubated with Dako swine anti-rabbit antibody (1:50, 28 min/ 37°C). Staining was amplified and detected by incubation with Ventana Streptavidin ABC system (16 min/

  • Immunohistochemical analysis of formaldehyde fixed human cephalic sections. Primary antibody ab10558 to CD45 incubated at a concentration of 1/100 for 4°C for 18 hours. Secondary antibody used was a goat anti-rabbit congugated to biotin at a 1/200 dilution. Blocking was done with serum at a 10% concentration for 1 hour at 25°C.

    See Abreview

  • Overlay histogram showing Jurkat cells stained with ab10558 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10558, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/1000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-CD45 antibody (ab10558) 使用論文

This product has been referenced in:
  • Hatzistergos KE  et al. S-Nitrosoglutathione Reductase Deficiency Enhances the Proliferative Expansion of Adult Heart Progenitors and Myocytes Post Myocardial Infarction. J Am Heart Assoc 4:N/A (2015). IHC-P ; Mouse . Read more (PubMed: 26178404) »
  • Eirin A  et al. Intrarenal Delivery of Mesenchymal Stem Cells and Endothelial Progenitor Cells Attenuates Hypertensive Cardiomyopathy in Experimental Renovascular Hypertension. Cell Transplant 24:2041-53 (2015). Flow Cyt ; Pig . Read more (PubMed: 25420012) »

See all 31 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rabbit Cell lysate - whole cell (rabbit Reticulocyte)
Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MES buffer))
Loading amount 0.5 µg
Specification rabbit Reticulocyte
Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Nov 23 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Injured spinal cord)
Antigen retrieval step None
Permeabilization Yes - Triton-X100
Specification Injured spinal cord
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative Paraformaldehyde
Username

清隆 新井

Verified customer

投稿 Sep 26 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Tonsil)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer ph6.0
Permeabilization No
Specification Tonsil
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 26°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 01 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Fukomys anselli Tissue sections (Spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris - high pH
Permeabilization Yes - 0.2% Triton-X
Specification Spleen
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Dr. Pascal Malkemper

Verified customer

投稿 Aug 09 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Heart)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization Yes - Triton
Specification Heart
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 20% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 04 2016

Abreviews
Application Western blot
Sample Rat Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount 14 µg
Specification Intestinal tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 11 2016

Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount 14 µg
Specification Intestinal tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 27 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF7)
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount 20 µg
Specification MCF7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 27 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (head and neck cancer)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer, pH6.0
Permeabilization No
Specification head and neck cancer
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 10 2015

Application Immunocytochemistry
Sample Human Cell (Whole blood(breast cancer patients in stage IV))
Permeabilization No
Specification Whole blood(breast cancer patients in stage IV)
Fixative Formaldehyde
Username

Mr. Chang Su Jeon

Verified customer

投稿 Jul 24 2015

1-10 of 57 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"