製品の概要

  • 製品名Anti-CD44 antibody [T2-F4]
    CD44 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [T2-F4] to CD44
  • アプリケーション適用あり: Flow Cyt, IP, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Con A-activated mouse LN and thymus cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab40983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
IP
ICC/IF
IHC-P
  • 追加情報Flow Cyt: Use 5-10µl for 106 cells.
    IP: Use at an assay dependent dilution.
    May also be used in antibody mediated cytotoxicity.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
    • 組織特異性Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
    • 配列類似性Contains 1 Link domain.
    • ドメインThe lectin-like LINK domain is responsible for hyaluronan binding.
    • 翻訳後修飾Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
      N-glycosylated.
      O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
      Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • LHR antibody
      • BA-1 antibody
      • CD 44 antibody
      • CD44 antibody
      • CD44 antigen antibody
      • CD44 molecule (Indian blood group) antibody
      • CD44 molecule antibody
      • CD44_HUMAN antibody
      • CDw44 antibody
      • Cell surface glycoprotein CD44 antibody
      • chondroitin sulfate proteoglycan 8 antibody
      • CSPG8 antibody
      • ECMR-III antibody
      • Epican antibody
      • Extracellular matrix receptor III antibody
      • GP90 lymphocyte homing/adhesion receptor antibody
      • HCELL antibody
      • hematopoietic cell E- and L-selectin ligand antibody
      • Heparan sulfate proteoglycan antibody
      • Hermes antigen antibody
      • homing function and Indian blood group system antibody
      • HSA antibody
      • HUTCH-I antibody
      • HUTCH1 antibody
      • Hyaluronate receptor antibody
      • IN antibody
      • MC56 antibody
      • MDU2 antibody
      • MDU3 antibody
      • MGC10468 antibody
      • MIC4 antibody
      • MUTCH1 antibody
      • PGP-1 antibody
      • PGP-I antibody
      • PGP1 antibody
      • Phagocytic glycoprotein 1 antibody
      • Phagocytic glycoprotein I antibody
      see all

    Anti-CD44 antibody [T2-F4] 画像

    • ab40983 staining CD44 in Human OSCC tongue tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% serum + 0.1% BSA for 30 minutes at 21°C.  Samples were incubated with primary antibody (1/100 in blocking buffer) for 2 hours at 21°C. An Alexa Fluor®488-conjugated Goat anti-rat IgG monoclonal(1/400) was used as the secondary antibody.

      See Abreview

    • ab40983 staining mouse embryonic stem cells by Flow Cytometery. Cells were fixed in paraformaldehyde and gating was done on live cells. The primary antibody was diluted 1/100 and incubated with sample for 30 minutes at 25°C. A PE conjugated goat polyclonal to rat IgG, diluted 1/100 was used as secondary.

      See Abreview

    • ab40983 used in Flow Cytometry.
      HUCPVCs were fixed in paraformaldehyde.
      ab40983 used at a 1/100 dilution.
      Secondary used was a goat anti-rat polyclonal conjugated to PE, used at a 1/100 dilution.

      Purple plot shows rat IgG isotype control and secondary antibody
      Green plot shows ab40983 and secondary antibody

      See Abreview

    • ab40983 staining CD44 protein on mutant mouse embryonic stem cell by ICC/IF. The cells were fixed in 1:1 methanol:acetone and blocked in 0.1% BSA plus 1% serum, for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the cells for 2 hours at 21°C. An Alexa Fluor® 488 conjugated goat anti-rat IgG, diluted 1/200, was used as the secondary antibody.

      See Abreview

    Anti-CD44 antibody [T2-F4] (ab40983) 使用論文

    ab40983 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step 1% serum + 0.1% BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
    Sample Human Cell (oral cancer cell line)
    Specification oral cancer cell line
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Ms. Zahra Abdalla

    Verified customer

    投稿 Feb 25 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (OSCC tongue)
    Specification OSCC tongue
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No
    Blocking step 0.1% BSA and 1% serum as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 21°C
    Username

    Ms. Zahra Abdalla

    Verified customer

    投稿 Apr 24 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (MDA-MB-231)
    Specification MDA-MB-231
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step 0.1% BSA + 1% goat serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Jan 11 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Mutant mouse embryonic stem cell)
    Specification Mutant mouse embryonic stem cell
    Fixative methanol:acetone 1:1
    Permeabilization No
    Blocking step 1% serum+0.1%BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 24 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (HUCPVCs)
    Specification HUCPVCs
    Preparation Cell harvesting/tissue preparation method: Cell dissociation buffer
    Sample buffer: Enzyme free
    Fixation Paraformaldehyde
    Permeabilization No
    Gating Strategy Live cells
    Username

    Abcam user community

    Verified customer

    投稿 Nov 15 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Mouse Cell (mutant embryonic stem cell)
    Specification mutant embryonic stem cell
    Preparation Cell harvesting/tissue preparation method: cell dissociation buffer
    Sample buffer: enzyme free
    Fixation Paraformaldehyde
    Permeabilization No
    Gating Strategy live cells
    Username

    Abcam user community

    Verified customer

    投稿 Nov 14 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Mouse Cell (embryonic stem)
    Specification embryonic stem
    Fixation Paraformaldehyde
    Permeabilization No
    Gating Strategy live cells
    Username

    Abcam user community

    Verified customer

    投稿 Jan 25 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"