製品の概要

  • 製品名Anti-CD44 antibody [MEM-263]
    CD44 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-263] to CD44
  • アプリケーション適用あり: IP, WB, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    COS-7 cells.

  • エピトープextracellular (N-terminal) domain
  • ポジティブ・コントロール
    • KG1a leukemia acute myeloid cell line This antibody gave a positive result in IHC in the following FFPE tissue: Human Skin Melanoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 2 µg/ml.
WB Use a concentration of 2 µg/ml. Use under non reducing condition.
Flow Cyt Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • 組織特異性Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • 配列類似性Contains 1 Link domain.
  • ドメインThe lectin-like LINK domain is responsible for hyaluronan binding.
  • 翻訳後修飾Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • LHR antibody
    • BA-1 antibody
    • CD 44 antibody
    • CD44 antibody
    • CD44 antigen antibody
    • CD44 molecule (Indian blood group) antibody
    • CD44 molecule antibody
    • CD44_HUMAN antibody
    • CDw44 antibody
    • Cell surface glycoprotein CD44 antibody
    • chondroitin sulfate proteoglycan 8 antibody
    • CSPG8 antibody
    • ECMR-III antibody
    • Epican antibody
    • Extracellular matrix receptor III antibody
    • GP90 lymphocyte homing/adhesion receptor antibody
    • HCELL antibody
    • hematopoietic cell E- and L-selectin ligand antibody
    • Heparan sulfate proteoglycan antibody
    • Hermes antigen antibody
    • homing function and Indian blood group system antibody
    • HSA antibody
    • HUTCH-I antibody
    • HUTCH1 antibody
    • Hyaluronate receptor antibody
    • IN antibody
    • MC56 antibody
    • MDU2 antibody
    • MDU3 antibody
    • MGC10468 antibody
    • MIC4 antibody
    • MUTCH1 antibody
    • PGP-1 antibody
    • PGP-I antibody
    • PGP1 antibody
    • Phagocytic glycoprotein 1 antibody
    • Phagocytic glycoprotein I antibody
    see all

Anti-CD44 antibody [MEM-263] 画像

  • IHC image of CD44 staining in Human Skin Melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9524, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Human peripheral blood lymphocytes stained with ab9524 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab9524, 0.1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

  • Western blotting of HPB-ALL cell lysate (non-reduced sample) stained by ab9524.

    Western blotting of HPB-ALL cell lysate (non-reduced sample) stained by ab9524.

Anti-CD44 antibody [MEM-263] (ab9524) 使用論文

This product has been referenced in:
  • Zhang L  et al. The extent of inflammatory infiltration in primary cancer tissues is associated with lymphomagenesis in immunodeficient mice. Sci Rep 5:9447 (2015). IHC-P . Read more (PubMed: 25819560) »
  • Samy M  et al. Loss of the malignant phenotype of human neuroblastoma cells by a catalytically inactive dominant-negative hTERT mutant. Mol Cancer Ther 11:2384-93 (2012). Human . Read more (PubMed: 22933702) »

See all 3 Publications for this product

Product Wall

Thank your for your recent enquiry regarding CD44 antibody ab9524. Unfortunately the epitope that this antibody reacts with is not known, we only know it reacts with the extracellular (N terminal) domain of cd44. Unfortunately I have been unable to...

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Ab9524 works poorly in reducing conditions but gives superb results in non-reducing conditions. I hope this information helps,

Thank your for your recent enquiry regarding CD44 antibody ab9524. Unfortunately the epitope that this antibody reacts with is not known, we only know it reacts with the extracellular (N terminal) domain of the protein. My apologies for the inconvenie...

Read More

We only know it reacts with extracellular (N terminal) domain.

The epitope is extracellular

The epitope that this antibody reacts with is not known.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"