製品の概要

  • 製品名Anti-CD44 antibody [MAC329]
    CD44 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [MAC329] to CD44
  • 特異性ab23844 recognises CD44.
  • アプリケーション適用あり: Flow Cyt, IHC-Fr, IHC-P, IPmore details
  • 種交差性
    交差種: Sheep, Goat, Horse, Cow, Dog, Pig
  • 免疫原

    Full length native protein: Purified porcine CD44

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab23844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
IHC-Fr
IHC-P
IP
  • 追加情報FACS: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
    • 組織特異性Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
    • 配列類似性Contains 1 Link domain.
    • ドメインThe lectin-like LINK domain is responsible for hyaluronan binding.
    • 翻訳後修飾Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
      N-glycosylated.
      O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
      Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • LHR antibody
      • BA-1 antibody
      • CD 44 antibody
      • CD44 antibody
      • CD44 antigen antibody
      • CD44 molecule (Indian blood group) antibody
      • CD44 molecule antibody
      • CD44_HUMAN antibody
      • CDw44 antibody
      • Cell surface glycoprotein CD44 antibody
      • chondroitin sulfate proteoglycan 8 antibody
      • CSPG8 antibody
      • ECMR-III antibody
      • Epican antibody
      • Extracellular matrix receptor III antibody
      • GP90 lymphocyte homing/adhesion receptor antibody
      • HCELL antibody
      • hematopoietic cell E- and L-selectin ligand antibody
      • Heparan sulfate proteoglycan antibody
      • Hermes antigen antibody
      • homing function and Indian blood group system antibody
      • HSA antibody
      • HUTCH-I antibody
      • HUTCH1 antibody
      • Hyaluronate receptor antibody
      • IN antibody
      • MC56 antibody
      • MDU2 antibody
      • MDU3 antibody
      • MGC10468 antibody
      • MIC4 antibody
      • MUTCH1 antibody
      • PGP-1 antibody
      • PGP-I antibody
      • PGP1 antibody
      • Phagocytic glycoprotein 1 antibody
      • Phagocytic glycoprotein I antibody
      see all

    Anti-CD44 antibody [MAC329] 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of canine tonsil tissue section labeling CD44  with ab23844 at 1/25 dilution. Tissue was fixed in formalin, heat mediated antigen retrieval wasperformed. Samples were incubated with primary antibody (1/25) for 1 hour. A HRP-conjugated rat ultramap polyclonal was used as the secondary antibody.

      See Abreview

    Anti-CD44 antibody [MAC329] (ab23844) 使用論文

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Dog Tissue sections (Tonsil)
    Antigen retrieval step Heat mediated
    Permeabilization No
    Specification Tonsil
    Fixative Formalin
    Username

    Mr. Emmanuel Bouchaert

    Verified customer

    投稿 Oct 06 2016

    Thank you for your enquiry. There is no concentration available for ab23844 as the antibody is provided in an unpurified form, tissue culture supernatant, and an exact concentration cannot be determined. Please contact us again if you have any addit...

    Read More

    You recently spoke with my colleague Maud Osborne regarding the use of a number of anti CD antibodies in IHC on paraffin embedded rat sections. As you have seen, none of the antibodies had been tested in the exact conditions you wish to try although my...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"