製品の概要

  • 製品名Anti-CD44 antibody [KM201]
    CD44 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [KM201] to CD44
  • アプリケーション適用あり: IP, WB, Flow Cyt, IHC-FoFr, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • エピトープab25340 reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule.
  • 特記事項


    ab25340 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and WhitlockWitte Cultures, prevent the earliest intrathymic precursors from homing to the thymus and costimulate the activation of freshly purified splenic CD4+ T cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 85 kDa.
Flow Cyt Use at an assay dependent concentration. ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-FoFr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent dilution.

ターゲット情報

  • 機能Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • 組織特異性Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • 配列類似性Contains 1 Link domain.
  • ドメインThe lectin-like LINK domain is responsible for hyaluronan binding.
  • 翻訳後修飾Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • LHR antibody
    • BA-1 antibody
    • CD 44 antibody
    • CD44 antibody
    • CD44 antigen antibody
    • CD44 molecule (Indian blood group) antibody
    • CD44 molecule antibody
    • CD44_HUMAN antibody
    • CDw44 antibody
    • Cell surface glycoprotein CD44 antibody
    • chondroitin sulfate proteoglycan 8 antibody
    • CSPG8 antibody
    • ECMR-III antibody
    • Epican antibody
    • Extracellular matrix receptor III antibody
    • GP90 lymphocyte homing/adhesion receptor antibody
    • HCELL antibody
    • hematopoietic cell E- and L-selectin ligand antibody
    • Heparan sulfate proteoglycan antibody
    • Hermes antigen antibody
    • homing function and Indian blood group system antibody
    • HSA antibody
    • HUTCH-I antibody
    • HUTCH1 antibody
    • Hyaluronate receptor antibody
    • IN antibody
    • MC56 antibody
    • MDU2 antibody
    • MDU3 antibody
    • MGC10468 antibody
    • MIC4 antibody
    • MUTCH1 antibody
    • PGP-1 antibody
    • PGP-I antibody
    • PGP1 antibody
    • Phagocytic glycoprotein 1 antibody
    • Phagocytic glycoprotein I antibody
    see all

Anti-CD44 antibody [KM201] 画像

  • Effect of CD44 blockade on hematopoiesis.
    Long term bone marrow cultures from Has1/2/3 triple knock-out mice were treated with 300 µM 4MU or vehicle control during weekly feedings. Where indicated, 100 µg/ml HMW HA (UC, 600-1,500 kD) or 10 µg/ml CD44-specific antibody (ab25340) were added.
  • Flow Cytometry analysis of BALB/c mouse bone marrow cells labeling CD44 with ab25340 at 1 μg/106 cells dilution (purple). A Mouse Anti-Rat IgG1-FITC was used as the secondary antibody. Grey - Isotype Control, Rat IgG1-UNLB, followed by Mouse Anti-Rat IgG1-FITC.

Anti-CD44 antibody [KM201] (ab25340) 使用論文

This product has been referenced in:
  • Donnelly JM  et al. Mesenchymal stem cells induce epithelial proliferation within the inflamed stomach. Am J Physiol Gastrointest Liver Physiol 306:G1075-88 (2014). Read more (PubMed: 24789207) »
  • Goncharova V  et al. Hyaluronan expressed by the hematopoietic microenvironment is required for bone marrow hematopoiesis. J Biol Chem 287:25419-33 (2012). Blocking ; Mouse . Read more (PubMed: 22654110) »

See all 9 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Mouse Tissue sections (spleen)
Specification spleen
Permeabilization No
Fixative Paraformaldehyde
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投稿 Jan 08 2014

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I am very pleased to hear you would like to accept our offer and test ab25340 in Human and IHC-Fr. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem th...

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I am sorry I have over-read your main question. We state only mouse as a tested species on the datasheet so far. All tested applicationsare specified on our datasheets, and these are updated as soon as any ...

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Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information. This information is stated on the datasheet in the immunogen section.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (T-cell lymphoma)
Specification T-cell lymphoma
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
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投稿 Apr 24 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"