Anti-CD44 抗体 [F10-44-2] (PE/Cy7 ®) (ab46793)

製品の概要

  • 製品名
    Anti-CD44 antibody [F10-44-2] (PE/Cy7 ®)
    CD44 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [F10-44-2] to CD44 (PE/Cy7 ®)
  • 標識
    PE/Cy7 ®. Ex: 496nm, Em: 774nm
  • アプリケーション
    適用あり: ICC/IF, Flow Cyt, IHC-Fr, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Purified human T cells

  • 特記事項

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab46793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200. See Abreview.
Flow Cyt 1/10 - 1/100. ab103534-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • 組織特異性
    Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • 配列類似性
    Contains 1 Link domain.
  • ドメイン
    The lectin-like LINK domain is responsible for hyaluronan binding.
  • 翻訳後修飾
    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • LHR antibody
    • BA-1 antibody
    • CD 44 antibody
    • CD44 antibody
    • CD44 antigen antibody
    • CD44 molecule (Indian blood group) antibody
    • CD44 molecule antibody
    • CD44_HUMAN antibody
    • CDw44 antibody
    • Cell surface glycoprotein CD44 antibody
    • chondroitin sulfate proteoglycan 8 antibody
    • CSPG8 antibody
    • ECMR-III antibody
    • Epican antibody
    • Extracellular matrix receptor III antibody
    • GP90 lymphocyte homing/adhesion receptor antibody
    • HCELL antibody
    • hematopoietic cell E- and L-selectin ligand antibody
    • Heparan sulfate proteoglycan antibody
    • Hermes antigen antibody
    • homing function and Indian blood group system antibody
    • HSA antibody
    • HUTCH-I antibody
    • HUTCH1 antibody
    • Hyaluronate receptor antibody
    • IN antibody
    • INLU-related p80 Glycoprotein antibody
    • MC56 antibody
    • MDU2 antibody
    • MDU3 antibody
    • MGC10468 antibody
    • MIC4 antibody
    • MUTCH1 antibody
    • PGP-1 antibody
    • PGP-I antibody
    • PGP1 antibody
    • Phagocytic glycoprotein 1 antibody
    • Phagocytic glycoprotein I antibody
    • Soluble CD44 antibody
    see all

画像

  • ab46793 staining CD44 in Human pancreatic adenocarcinoma cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA for 30 minutes at 24°C. Samples were incubated with primary antibody 1/200 (1% BSA in PBS)  for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG, dilution 1/300, was used as secondary antibody.

    See Abreview

  • Flow Cytometry analysis of Human peripheral blood lymphocytes labeling CD44 with ab46793 at 10 μL/106 cells (purple). Grey - Isotype Control, Mouse IgG2a-PE/CY7.

  • Overlay histogram showing peripheral blood lymphocytes stained with ab46793 (red line). The cells were incubated with the antibody (ab46793, 1/100) for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) with secondary antibody DyLight® 649 goat anti-mouse IgG (H&L) (ab96882) at 1/200 dilution for 30 min at 4ºC. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.

参考文献

This product has been referenced in:
  • Zhao F  et al. Programmed cell death 1 correlates with the occurrence and development of MG63 osteosarcoma. Oncol Lett 12:5199-5204 (2016). Flow Cyt ; Human . Read more (PubMed: 28105229) »
  • Bustos ML  et al. Activation of human mesenchymal stem cells impacts their therapeutic abilities in lung injury by increasing interleukin (IL)-10 and IL-1RN levels. Stem Cells Transl Med 2:884-95 (2013). Human . Read more (PubMed: 24089414) »

See all 5 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Pancreatic adenocarcinoma)
Specification
Pancreatic adenocarcinoma
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Dr. Jennifer Dembinski

Verified customer

投稿 Nov 17 2009

Vielen Dank für die Anfrage. Dass ab46793 nicht auf der Liste auftaucht, bedeutet dass er nicht getestet wurde, ob er mit Maus kreuzreagiert. In der Tat ist ab46793 für die Immunhistochemie (IHC-P/Fr - neben anderen Anwendungen wie Durchflusszy...

Read More

Dein Suchergebnis kann ich leider bestätigen: Wir haben derzeit keinen anti-human-nicht-Maus-kreuzreaktiven CD44- Antikörper erhältlich. Tut mir leid, dass ich keine besseren Nachrichten habe. Für weitere Fragen stehe ich jederzeit gerne zur Verfügung.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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