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RabMAb

Anti-CD44 抗体 [EPR1013Y] (ab51037)

製品の概要

  • 製品名
    Anti-CD44 antibody [EPR1013Y]
    CD44 一次抗体 製品一覧
  • 製品の詳細
    Rabbit Monoclonal [EPR1013Y] to CD44
  • アプリケーション
    適用あり: IHC-P, IHC-Fr, WB, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CD44 aa 150-250. The exact sequence is proprietary.

  • ポジティブ・コントロール
    • TF-1 cell lysate or human urinary bladder tissue.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
WB 1/5000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
    • 組織特異性
      Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
    • 配列類似性
      Contains 1 Link domain.
    • ドメイン
      The lectin-like LINK domain is responsible for hyaluronan binding.
    • 翻訳後修飾
      Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
      N-glycosylated.
      O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
      Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • LHR antibody
      • BA-1 antibody
      • CD 44 antibody
      • CD44 antibody
      • CD44 antigen antibody
      • CD44 molecule (Indian blood group) antibody
      • CD44 molecule antibody
      • CD44_HUMAN antibody
      • CDw44 antibody
      • Cell surface glycoprotein CD44 antibody
      • chondroitin sulfate proteoglycan 8 antibody
      • CSPG8 antibody
      • ECMR-III antibody
      • Epican antibody
      • Extracellular matrix receptor III antibody
      • GP90 lymphocyte homing/adhesion receptor antibody
      • HCELL antibody
      • hematopoietic cell E- and L-selectin ligand antibody
      • Heparan sulfate proteoglycan antibody
      • Hermes antigen antibody
      • homing function and Indian blood group system antibody
      • HSA antibody
      • HUTCH-I antibody
      • HUTCH1 antibody
      • Hyaluronate receptor antibody
      • IN antibody
      • INLU-related p80 Glycoprotein antibody
      • MC56 antibody
      • MDU2 antibody
      • MDU3 antibody
      • MGC10468 antibody
      • MIC4 antibody
      • MUTCH1 antibody
      • PGP-1 antibody
      • PGP-I antibody
      • PGP1 antibody
      • Phagocytic glycoprotein 1 antibody
      • Phagocytic glycoprotein I antibody
      • Soluble CD44 antibody
      see all

    Anti-CD44 antibody [EPR1013Y] 画像

    • ab51037 (1:100) showing positive staining in human Breast carcinoma tissue.

    • ab51037 used at a 1/200 dilution (red) to detect CD44 in human MDA-MB-231 cells by Flow Cytometry. The secondary used was a Pgoat anti-rabbit IgG (Alexa Fluor® 488) polyclonal used at a 1/2000 dilution. Isotype control (black): Rabbit monoclonal IgG (ab172730). Unlabelled control (Blue): Cell without incubation with primary antibody and secondary antibody.

    • Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    • All lanes : Anti-CD44 antibody [EPR1013Y] (ab51037) at 1/1000 dilution

      Lane 1 : MCF-7 whole cell lysate
      Lane 2 : Jurkat whole cell lysate
      Lane 3 : MDA-MB-231 whole cell lysate
      Lane 4 : HeLa whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 82 kDa
      Observed band size : 81 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and diluting buffer 5% NFDM/TBST

      The expression of CD44 in MCF-7 is low (PMID: 25635866; PMID: 26005723). Jurkat does not express CD44 (PMID: 24127558).

    • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    • Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    • Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    • Anti-CD44 antibody [EPR1013Y] (ab51037) at 1/5000 dilution + TF-1 cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled. at 1/2000 dilution

      Predicted band size : 82 kDa
      Observed band size : 82 kDa
    • ab51037 (1:100) showing positive staining in human Glioma tissue.

    • ab51037 (1:100) showing positive staining in human Thyroid gland carcinoma tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-CD44 antibody [EPR1013Y] (ab51037) 使用論文

    This product has been referenced in:
    • Edelman R  et al. Hyaluronic acid-serum albumin conjugate-based nanoparticles for targeted cancer therapy. Oncotarget 8:24337-24353 (2017). Read more (PubMed: 28212584) »
    • Saad A  et al. Adipose-derived mesenchymal stem cells from patients with atherosclerotic renovascular disease have increased DNA damage and reduced angiogenesis that can be modified by hypoxia. Stem Cell Res Ther 7:128 (2016). Flow Cyt ; Human . Read more (PubMed: 27612459) »

    See all 32 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HepG2 liver cancer cell line)
    Permeabilization
    Yes - 0.1% Triton X100 in PBS
    Specification
    HepG2 liver cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Sep 21 2017


    According to SwissProt, the immunogen range indicates it can detect most isoforms, excluding only isoform 2 and 19.

    http://www.uniprot.org/uniprot/P16070


    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jun 03 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela)
    Gel Running Conditions
    Non-reduced Denaturing (7.5% SDS-PAGE)
    Loading amount
    40 µg
    Specification
    Hela
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Sep 10 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
    Sample
    Mouse Tissue sections (Mouse 15.5dpc)
    Specification
    Mouse 15.5dpc
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 May 14 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Flow Cytometry
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Sample
    Mouse Cell (differentiating ES)
    Specification
    differentiating ES
    Gating Strategy
    live cells
    Preparation
    Cell harvesting/tissue preparation method: cell dissociation buffer
    Sample buffer: enzyme free
    Username

    Abcam user community

    Verified customer

    投稿 Jul 09 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Fixative
    Formalin
    Antigen retrieval step
    Other
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    投稿 Apr 12 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MB-MDA-231)
    Loading amount
    20 µg
    Specification
    MB-MDA-231
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Ms. Zahra Abdalla

    Verified customer

    投稿 Dec 19 2012

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody in mouse.

    I would like to reassure you that this antibody is tested and covered by our 6 month guarante...

    Read More

    Vielen Dank für Ihren Anruf.

    Leider handelt es sich bei diesem Produkt um einen Antikörper, der sich noch in dem ursprünglichen Zellkultur- Überstand befindet: dies macht eine Messung der Antikörperkonzentration aufgru...

    Read More

    1-10 of 31 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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