The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at a concentration of 20 µg/ml.
IP: Use at a concentration of 20 µg/ml.
WB: Use at a concentration of 1-4 µg/ml. Predicted molecular weight: 82 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Contains 1 Link domain.
The lectin-like LINK domain is responsible for hyaluronan binding.
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. N-glycosylated. O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
hematopoietic cell E- and L-selectin ligand antibody
Heparan sulfate proteoglycan antibody
Hermes antigen antibody
homing function and Indian blood group system antibody
Hyaluronate receptor antibody
INLU-related p80 Glycoprotein antibody
Phagocytic glycoprotein 1 antibody
Phagocytic glycoprotein I antibody
Soluble CD44 antibody
Anti-CD44 antibody 画像
Western blot - Anti-CD44 antibody (ab24504)
All lanes : Anti-CD44 antibody (ab24504) at 2 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 82 kDa Observed band size : 82 kDa Additional bands at : 25-50 kDa (possible isoform).
Immunohistochemistry (Frozen sections) - Anti-CD44 antibody (ab24504)Image from Dubrovska A et al., PLoS One. 2012;7(2):e31226. Epub 2012 Feb 16. Fig S2.; doi:10.1371/journal.pone.0031226; February 16, 2012, PLoS ONE 7(2): e31226.
Immunohistochemical analysis of Human prostate cancer cell xenograft tissue, staining CD44 (red) with ab24504.
Tissue was fixed with paraformaldehyde, permeabilized with 0.4% Triton X-100 and blocked with 10% serum. Samples were incubated overnight with primary antibody (1/100) overnight at 4°C. An AlexaFluor®555-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody.
Feng H et al. Involvement of Ubiquitin-Editing Protein A20 in Modulating Inflammation in Rat Cochlea Associated with Silver Nanoparticle-Induced CD68 Upregulation and TLR4 Activation. Nanoscale Res Lett11:240 (2016).
Read more (PubMed: 27142878) »
Kennedy E et al. Embryonic rat vascular smooth muscle cells revisited - a model for neonatal, neointimal SMC or differentiated vascular stem cells? Vasc Cell6:6 (2014).
Read more (PubMed: 24628920) »