Anti-CD44 抗体 [156-3C11] (Biotin) (ab79214)

製品の概要

  • 製品名Anti-CD44 antibody [156-3C11] (Biotin)
    CD44 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [156-3C11] to CD44 (Biotin)
  • 標識Biotin
  • 特異性ab79214 reacts with red cells pretreated with 2 aminoethylisothiouronium bromide (AET). Digestion of intact erythrocytes by trypsin treatment leaves a 50-54 kDa protein fragment attached to the membrane, while chymotrypsin leaves a somewhat similarly sized 51-56 kDa component that are recognized by ab79214. ab79214 enhances IL2 production by CD2 transfected murine hybridoma stimulated by JY cells while it has no significant effect on IL2 production by Jurkat cells stimulted with CD3, PMA, or CD2. ab79214 enhances proliferation of PBL stimulated with CD2. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human, Monkey, Baboon
  • 免疫原

    Stimulated human leukocytes.

  • エピトープResistant to digestion by trypsin and chymotrypsin
  • ポジティブ・コントロール
    • HUVEC or HeLa cells. Human tonsil or esophageal carcinoma tissues.
  • 特記事項


    Key reference: This product has been used in: Schlossman SF, et. al. Leucocyte Typing V, p1713-1719, Oxford Univ. Press, 1995.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 82 kDa.

For 2 hours at room temperature.

IP Use at 2 µg/mg of lysate.

Native verified, use Protein A.

IHC-P Use a concentration of 1 - 2 µg/ml.

For 30 minutes at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Flow Cyt Use at an assay dependent concentration. ab97679-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • 組織特異性Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • 配列類似性Contains 1 Link domain.
  • ドメインThe lectin-like LINK domain is responsible for hyaluronan binding.
  • 翻訳後修飾Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • LHR antibody
    • BA-1 antibody
    • CD 44 antibody
    • CD44 antibody
    • CD44 antigen antibody
    • CD44 molecule (Indian blood group) antibody
    • CD44 molecule antibody
    • CD44_HUMAN antibody
    • CDw44 antibody
    • Cell surface glycoprotein CD44 antibody
    • chondroitin sulfate proteoglycan 8 antibody
    • CSPG8 antibody
    • ECMR-III antibody
    • Epican antibody
    • Extracellular matrix receptor III antibody
    • GP90 lymphocyte homing/adhesion receptor antibody
    • HCELL antibody
    • hematopoietic cell E- and L-selectin ligand antibody
    • Heparan sulfate proteoglycan antibody
    • Hermes antigen antibody
    • homing function and Indian blood group system antibody
    • HSA antibody
    • HUTCH-I antibody
    • HUTCH1 antibody
    • Hyaluronate receptor antibody
    • IN antibody
    • MC56 antibody
    • MDU2 antibody
    • MDU3 antibody
    • MGC10468 antibody
    • MIC4 antibody
    • MUTCH1 antibody
    • PGP-1 antibody
    • PGP-I antibody
    • PGP1 antibody
    • Phagocytic glycoprotein 1 antibody
    • Phagocytic glycoprotein I antibody
    see all

Anti-CD44 antibody [156-3C11] (Biotin) (ab79214) 使用論文

ab79214 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela cell)
Specification Hela cell
Fixative Paraformaldehyde
Permeabilization Yes - 0.03% Triton X 100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

投稿 Jan 16 2013

Thank you for your call yesterday and for your patience while I have looked through the literature regarding your enquiry.

I have found a reference that used the CD44 antibody, clone F10-44-2 to immunoprecipitate HIV-1 particles (see attached...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unserem Wissen nach wurde ab79214 bisher noch nichtmit murinenProben getestet und wir können keine Aussage darüber treffen ob er nicht bindet, wie vo...

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Vielen Dank für Ihre Anfrage.

Leider muss ich Ihnen mitteilen, dass ab79214 nicht für Ihre Versuche geeignet ist. ab79214 ist getestet und auch garantiert für humane Proben. Allerding wissen wir nicht ob Mausproben auch erkannt...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"