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Stimulated human leukocytes.
Key reference: This product has been used in: Schlossman SF, et. al. Leucocyte Typing V, p1713-1719, Oxford Univ. Press, 1995.
Our Abpromise guarantee covers the use of ab79214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 82 kDa.
For 2 hours at room temperature.
|IP||Use at 2 µg/mg of lysate.
Native verified, use Protein A.
|IHC-P||Use a concentration of 1 - 2 µg/ml.
For 30 minutes at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
|Flow Cyt||Use at an assay dependent concentration. ab97679-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration.|
ab79214 has not yet been referenced specifically in any publications.