製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CD42d
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Horse, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human CD42d.

    (Peptide available as ab74425.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Lysates: EBS E14 TG2A Day 8 Whole Cell, Raji Whole Cell, EBS E14 TG2A Day 0 Whole Cell, EBS E14 TG2A Day 5 Whole Cell

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab65017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 61 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis.
  • 組織特異性
    Platelets and megakaryocytes.
  • 配列類似性
    Contains 13 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • 翻訳後修飾
    The N-terminus is blocked.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 42d antibody
    • CD42d antibody
    • CD42d antigen antibody
    • Glycoprotein 5 antibody
    • Glycoprotein V antibody
    • Glycoprotein V platelet antibody
    • GlycoproteinV antibody
    • Gp 5 antibody
    • GP V antibody
    • GP5 antibody
    • GPV antibody
    • GPV_HUMAN antibody
    • Platelet glycoprotein V antibody
    • Platelet glycoprotein V precursor antibody
    see all

画像

  • All lanes : Anti-CD42d antibody (ab65017) at 1 µg/ml

    Lane 1 : EBS E14 TG2A Day 8 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : EBS E14 TG2A Day 0 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate
    Lane 4 : EBS E14 TG2A Day 5 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 61 kDa
    Observed band size: 82 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes


    CD42d has a predicted molecular weight of 61 kDa but contains a number of potential glycosylation sites (SwissProt). We believe that the observed 81 kDa band correlates to the glycosylated form of the uncleaved protein. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

参考文献

ab65017 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Rat Tissue lysate - whole (brain tissue)
Gel Running Conditions
Reduced Denaturing (Bio-rad Mini-PROTEAN® TGX™ Precast Gels)
Loading amount
20 µg
Specification
brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Feb 28 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (hepatocytes)
Permeabilization
Yes - 0.1% Triton X-100
Specification
hepatocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

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Verified customer

投稿 Dec 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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