製品の概要

  • 製品名Anti-CD42b antibody
    CD42b 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CD42b
  • アプリケーション適用あり: IHC-Fr, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cat
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 573-622 (RGSLPTFRSS LFLWVRPNGR VGPLVAGRRP SALSQGRGQD LLSTVSIRYS) of Human CD42b (NP_000164)

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab104704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/1000.
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性CD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor. It interacts with extracellular matrix as well as with adhesion molecules. Its expression and activation seems to be regulated by VEGF and PDGF.
  • 細胞内局在Cell Membrane
  • 参照データベース
  • 別名
    • Antigen CD42b alpha antibody
    • BDPLT1 antibody
    • BDPLT3 antibody
    • BSS antibody
    • CD 42b antibody
    • CD42b alpha antibody
    • CD42B antibody
    • CD42b antigen antibody
    • DBPLT3 antibody
    • GLYCOCALICIN antibody
    • Glycoprotein Ib (platelet) alpha polypeptide antibody
    • Glycoprotein Ibalpha antibody
    • GP Ib alpha antibody
    • GP1B antibody
    • GP1BA antibody
    • GPIb alpha antibody
    • GPIbA antibody
    • MGC34595 antibody
    • Platelet glycoprotein Ib alpha chain antibody
    • Platelet glycoprotein Ib alpha polypeptide antibody
    • Platelet membrane glycoprotein 1b alpha subunit antibody
    • VWDP antibody
    see all

Anti-CD42b antibody 画像

  • IHC-Fr image of CD42b staining on human blood film using ab104704 (1:1000). The blood was fixed uisng paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. Blocking was performed using 10 % Donkey serum for 1 hour at 24°C. The primary antibody was diluted 1:1000 using 0.1% TritonX with 0.1x PBS and 10% Donkey serum; incubated for 4 hours at 24°C. The Secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000)

    See Abreview

  • Anti-CD42b antibody (ab104704) at 0.25 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size : 69 kDa
    Gel concentration: 12%

Anti-CD42b antibody (ab104704) 使用論文

ab104704 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (liver cancer)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrite buffer, pH6.0
Permeabilization No
Specification liver cancer
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
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投稿 Aug 19 2016

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Sample Mouse Tissue sections (platelets in fetal lung (E18.5))
Specification platelets in fetal lung (E18.5)
Permeabilization No
Fixative Paraformaldehyde
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投稿 Dec 03 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Human Tissue sections (Human blood film)
Specification Human blood film
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Jun 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (platelet)
Loading amount 20 µg
Specification platelet
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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投稿 Nov 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"