Anti-CD41/Integrin alpha 2b 抗体 (ab83961)

製品の概要

  • 製品名
    Anti-CD41/Integrin alpha 2b antibody
    CD41 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CD41/Integrin alpha 2b
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human CD41 aa 400-500 conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human CD41.

    (Peptide available as ab95922.)
    Database link: P08514
    (Peptide available as ab95922, ab95922)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysate: Human Platelet.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 113 kDa).

ターゲット情報

  • 機能
    Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
  • 組織特異性
    Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.
  • 関連疾患
    Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
  • 配列類似性
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • antigen CD41 antibody
    • BDPLT16 antibody
    • BDPLT2 antibody
    • CD41 antibody
    • CD41B antibody
    • form 2 antibody
    • GP2B antibody
    • GPalpha IIb antibody
    • GPIIb antibody
    • GT antibody
    • GTA antibody
    • HPA3 antibody
    • Integrin alpha 2b antibody
    • Integrin alpha IIb antibody
    • Integrin alpha-IIb light chain antibody
    • Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) antibody
    • ITA2B_HUMAN antibody
    • Itga2b antibody
    • ITGAB antibody
    • platelet fibrinogen receptor, alpha subunit antibody
    • platelet glycoprotein IIb of IIb/IIIa complex antibody
    • Platelet membrane glycoprotein IIb antibody
    • platelet specific antigen BAK antibody
    • PPP1R93 antibody
    see all

画像

  • Anti-CD41/Integrin alpha 2b antibody (ab83961) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 113 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa,80 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

プロトコール

参考文献

ab83961 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting Abcam.

The alignment between the human and porcine proteins is below our threshold for recommending cross reactivity with a 78% homology. I would therefore not recommend any product created from the full length prote...

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