製品の概要

  • 製品名Anti-CD41 antibody
    CD41 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CD41
  • 特異性Reacts with human 113 kDa CD41.
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: a wide range of other species
  • 免疫原

    Synthetic peptide corresponding to Human CD41 (N terminal). Synthetic peptide derived from the N terminal domain of Human CD41.
    Database link: P08514

  • 特記事項

    This product was changed from whole antiserum to Protein A purified on 15th June 2016. The following lots are from whole antiserum and are still in stock as of 15th June 2016, GR264406-3, GR250036-5 , GR250036-4. Lot numbers higher than GR264406-3, will be Protein A purified. Please note that the dilutions may need to be adjusted accordingly.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/5000. Predicted molecular weight: 113 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 20942599
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
  • 組織特異性Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.
  • 関連疾患Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
  • 配列類似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • antigen CD41 antibody
    • BDPLT16 antibody
    • BDPLT2 antibody
    • CD41 antibody
    • CD41B antibody
    • form 2 antibody
    • GP2B antibody
    • GPalpha IIb antibody
    • GPIIb antibody
    • GT antibody
    • GTA antibody
    • HPA3 antibody
    • Integrin alpha 2b antibody
    • Integrin alpha IIb antibody
    • Integrin alpha-IIb light chain antibody
    • Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) antibody
    • ITA2B_HUMAN antibody
    • Itga2b antibody
    • ITGAB antibody
    • platelet fibrinogen receptor, alpha subunit antibody
    • platelet glycoprotein IIb of IIb/IIIa complex antibody
    • Platelet membrane glycoprotein IIb antibody
    • platelet specific antigen BAK antibody
    • PPP1R93 antibody
    see all

Anti-CD41 antibody 画像

  • All lanes : Anti-CD41 antibody (ab63983) at 1/10000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 113 kDa
    Observed band size : 113 kDa


    Exposure time : 15 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-CD41 antibody (ab63983) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 113 kDa
    Observed band size : 113 kDa


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-CD41 antibody (ab63983) 使用論文

This product has been referenced in:
  • Anea CB  et al. Pulmonary platelet thrombi and vascular pathology in acute chest syndrome in patients with sickle cell disease. Am J Hematol 91:173-8 (2016). IHC-P ; Human . Read more (PubMed: 26492581) »
  • Yuan L & Liu X Platelets are associated with xenograft tumor growth and the clinical malignancy of ovarian cancer through an angiogenesis-dependent mechanism. Mol Med Rep 11:2449-58 (2015). IHC-P, IHC-Fr ; Human . Read more (PubMed: 25502723) »

See all 9 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Blood smear)
Specification Blood smear
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative Formaldehyde
Username

Mr. Simon Cleary

Verified customer

投稿 Feb 22 2016

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Hela, Hep G2)
Specification Hela, Hep G2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jan 05 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Hela, Hep G2)
Specification Hela, Hep G2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Dec 31 2014

Thank you for confirming those details. I have now arranged for a new vial of ab63983 to be sent to you free of charge. This is on the order number 1162584.

Please do not worry about the order number and lot number, I have this information to...

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Thank you for contacting us yesterday and reporting the problems encountered with the Anti-CD41/Integrin alpha 2b antibody (ab63983).

As discussed over the phone, I am able to offer you an alternative lot of ab63983 if you would like to try i...

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Thank you for getting back to me and sorry for the delay in my response.

I have been having some difficulty in finding further choices which may be suitable for your experiments due to the stringent constraints. We have a number of VCAM-1 ant...

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Thank you for contacting us and your interest in our products.

I have tried to find some alternative suggestions for your experiment that you may want to consider. I found no further antibodies against VE-Cadherin that might be suitable and a...

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I am writing to inquire if the heat induced antigen retrieval step has helped to improve the results? I would be pleased to receive an update on how these experiments are progressing.
If the antibody is still not working as stated on the datasheet...

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Thank you very much for your answer.
Following your answer on the antigen retrieval, I would like to confirm one more point. Indeed, have the slides been de-paraffinized by sequential incubations? If the slides have not been deparaffinized, this c...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monit...

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1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"