製品の概要
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製品名Anti-CD40L antibody [MR-1]
CD40L 一次抗体 製品一覧 -
製品の詳細Armenian Hamster monoclonal [MR-1] to CD40L
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特異性This antibody detects mouse CD154 / CD40L (CD40 ligand), a 39 kDa cell surface glycoprotein expressed predominantly on activated CD4+ lymphocytes.
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アプリケーション適用あり: IHC-P, IHC-Fr, IP, Functional Studies, ICC, Flow Cytmore details
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種交差性交差種: Mouse
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免疫原
Tissue/ cell preparation - activated mouse Th1 clone D1.6
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ポジティブ･コントロール
- 6-8 hour activated mouse splenocytes
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特記事項
Functional Application: Blocking T cell costimulation in vivo.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
精製度Protein A purified
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特記事項（精製）Purified from cell culture supernatant (> 95% by SDS-PAGE)
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一次抗体 備考Functional Application: Blocking T cell costimulation in vivo.
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ポリ/モノモノクローナル
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クローン名MR-1
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アイソタイプIgG1
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研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab99894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use at an assay dependent dilution. | |
| IHC-Fr | Use at an assay dependent dilution. | |
| IP | Use at an assay dependent dilution. | |
| Functional Studies | Use at an assay dependent dilution. | |
| ICC | Use at an assay dependent dilution. | |
| Flow Cyt | Use a concentration of 4 µg/ml. |
ターゲット情報
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機能Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. -
組織特異性Specifically expressed on activated CD4+ T-lymphocytes.
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関連疾患Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
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配列類似性Belongs to the tumor necrosis factor family.
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翻訳後修飾The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated. -
細胞内局在Secreted and Cell membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 21947 Mouse
- SwissProt: P27548 Mouse
- Unigene: 4861 Mouse
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別名
- CD 40L antibody
- CD154 antibody
- CD40 antigen ligand antibody
see all
プロトコール
Anti-CD40L antibody [MR-1] (ab99894) 使用論文
ab99894 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"