Anti-TRAP/CD40L 抗体 (ab65854)
Key features and details
- Rabbit polyclonal to TRAP/CD40L
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TRAP/CD40L antibody
TRAP/CD40L 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRAP/CD40L -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide corresponding to Human TRAP/CD40L (N terminal). Different from the relative mouse sequence by two amino acids
Database link: P29965 -
ポジティブ・コントロール
- IHC-P: Rat intestine, human mammary tissues. WB: MCF-7, HeLa, Jurkat, HMY2, COLO320 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65854の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa).
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特記事項 |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa). |
ターゲット情報
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機能
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. -
組織特異性
Specifically expressed on activated CD4+ T-lymphocytes. -
関連疾患
Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 959 Human
- Entrez Gene: 21947 Mouse
- Entrez Gene: 84349 Rat
- Omim: 300386 Human
- SwissProt: P29965 Human
- SwissProt: P27548 Mouse
- SwissProt: Q9Z2V2 Rat
- Unigene: 592244 Human
see all -
別名
- CD 40L antibody
- CD154 antibody
- CD40 antigen ligand antibody
see all
画像
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All lanes : Anti-TRAP/CD40L antibody (ab65854) at 0.5 µg/ml
Lane 1 : MCF-7 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : HMY2 whole cell lysate
Lane 5 : COLO320 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 29 kDa -
TRAP/CD40L was detected in paraffin-embedded section of human mammary tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65854 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab65854 は 12 報の論文で使用されています。
- Ma J et al. Role of HSP90α in osteoclast formation and osteoporosis development. Exp Ther Med 23:273 (2022). PubMed: 35251339
- Yang B et al. γ-Secretase inhibitors suppress IL-20-mediated osteoclastogenesis via Notch signalling and are affected by Notch2 in vitro. Scand J Immunol 96:e13169 (2022). PubMed: 35384009
- Ji W et al. Triptolide attenuates inhibition of ankylosing spondylitis-derived mesenchymal stem cells on the osteoclastogenesis through modulating exosomal transfer of circ-0110634. J Orthop Translat 36:132-144 (2022). PubMed: 36185580
- Jia X et al. Overexpression of miRNA-22-3p attenuates osteoporosis by targeting MAPK14. Exp Ther Med 22:692 (2021). PubMed: 33986857
- Feng Q et al. Denosumab inhibits MCF-7 cell line-induced spontaneous osteoclastogenesis via the RANKL/MALAT1/miR-124 axis. Transl Cancer Res 9:2482-2491 (2020). PubMed: 35117607