製品の概要

  • 製品名Anti-CD40L antibody
    CD40L 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CD40L
  • アプリケーション適用あり: Flow Cyt, ICC/IF, ELISA, WB, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide surrounding amino acid 246 of human CD40 Ligand

  • ポジティブ・コントロール
    • Human muscle tissue sections

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/200.
ICC/IF 1/200. See Abreview
ELISA Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/100. Fix with acetone -20°C for 10 min (provides a better signal than unfixed tissue; PFA 4% abolishes the signal). Incubate primary for 24h at 4°C. Use amplification (indirect IF).

ターゲット情報

  • 機能Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
    Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
  • 組織特異性Specifically expressed on activated CD4+ T-lymphocytes.
  • 関連疾患Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
  • 配列類似性Belongs to the tumor necrosis factor family.
  • 翻訳後修飾The soluble form derives from the membrane form by proteolytic processing.
    N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
    Not O-glycosylated.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 40L antibody
    • CD154 antibody
    • CD40 antigen ligand antibody
    • CD40 ligand antibody
    • CD40 ligand, soluble form antibody
    • CD40-L antibody
    • CD40L antibody
    • CD40L_HUMAN antibody
    • CD40LG antibody
    • gp39 antibody
    • hCD40L antibody
    • HIGM1 antibody
    • IGM antibody
    • IMD3 antibody
    • T B cell activating molecule antibody
    • T BAM antibody
    • T-cell antigen Gp39 antibody
    • TNF-related activation protein antibody
    • TNFSF5 antibody
    • TrAP antibody
    • Tumor necrosis factor (ligand) superfamily member 5 antibody
    • Tumor necrosis factor ligand superfamily member 5 antibody
    see all

Anti-CD40L antibody 画像

  • ab2391 at a 1/100 dilution staining CD40L from human inflamed muscle tissue sections by immunohistochemistry (frozen sections). The tissue was acetone fixed (this provides a better result than unfixed tissue) and then incubated with the antibody for 24 hours at 4 °C. Alexa-Fluor ® 594 was used as the secondary antibody.

    See Abreview

  • ab2391 at 1/500 staining rat spleen tissue sections by IHC-P. The tissue was fomraldehyde fixed and blocked with serum prior to incubation with the antibody for 18 hours. An Alexa-Fluor ® 488 conjugated goat anti-rabbit was used as the secondary.

    See Abreview

  • ab2391 staining CD40L in Human platelet cells by Flow cytometry.
    Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.

    See Abreview

Anti-CD40L antibody (ab2391) 使用論文

ab2391 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (RAW 264.7)
Specification RAW 264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 May 23 2012

Application Immunocytochemistry
Sample Human Cell (Monocytes from Apheresis)
Specification Monocytes from Apheresis
Fixative BD Cytofix/Cytoperm 20 min
Permeabilization Yes - BD Cytofix/Cytoperm 20 min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 2°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Feb 02 2012

Thank you for contacting Abcam regarding our CD40 and CD40L antibodies. Below please find the requested information: ab2391 Rabbit Polyclonal to CD40L - Unfortunately the specific immunogen sequence is proprietary, however I can inform you that...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Platelets)
Loading amount 20 µg
Specification Platelets
Treatment A23187 for 1 h
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Nov 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Platelets)
Specification Platelets
Preparation Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy Platelets
Username

Abcam user community

Verified customer

投稿 Dec 31 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (spinal cord homogenate)
Loading amount 20 µg
Specification spinal cord homogenate
Treatment sham or nerve transected
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Aug 06 2008

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spleen)
Specification spleen
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Ms. Nancy Nutile-McMenemy

Verified customer

投稿 Jul 11 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (spleen)
Specification spleen
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Ms. Nancy Nutile-McMenemy

Verified customer

投稿 Jul 06 2007

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