The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5-1µg for 105-8 cells. Staining the cell sample in a final volume of 100µl is recommended.
Receptor for TNFSF5/CD40LG.
B-cells and in primary carcinomas.
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
Flow cytometry staining of BALB/c splenocytes with 0.5 µg of Armenian Hamster IgG Isotype Control FITC (open histogram) or 0.5 µg of ab95535 (filled histogram). Total viable cells were used for analysis.
Ripoll È et al. CD40 gene silencing reduces the progression of experimental lupus nephritis modulating local milieu and systemic mechanisms. PLoS One8:e65068 (2013).
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Inaba K et al. The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro. J Exp Med180:1849-60 (1994).
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