製品の概要

  • 製品名Anti-CD40 antibody
    CD40 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CD40
  • 特異性The antibody specificity is confirmed by specific peptide inhibition in immunoblotting studies.
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Human, Pig, Monkey
    交差が予測される動物種: Chicken, Dog
  • 免疫原

    Synthetic peptide:

    CQPVTQEDGKESRISVQERQ

    conjugated to KLH, corresponding to amino acids 258-277 of Human CD40.

  • ポジティブ・コントロール
    • HeLa Cell Lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.
  • 特記事項Storage below -20°C is not recommended. For maximum product recovery centrifuge the product vial before removing cap. Shipped on gel packs.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).

ターゲット情報

  • 機能Receptor for TNFSF5/CD40LG.
  • 組織特異性B-cells and in primary carcinomas.
  • 関連疾患Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
  • 配列類似性Contains 4 TNFR-Cys repeats.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI326936 antibody
    • B cell associated molecule CD40 antibody
    • B cell surface antigen CD40 antibody
    • B cell-associated molecule antibody
    • B-cell surface antigen CD40 antibody
    • Bp50 antibody
    • CD 40 antibody
    • CD40 antibody
    • CD40 antigen (TNF receptor superfamily member 5) antibody
    • CD40 antigen antibody
    • CD40 molecule antibody
    • CD40 molecule, TNF receptor superfamily member 5 antibody
    • CD40 protein antibody
    • CD40 type II isoform antibody
    • CD40L receptor antibody
    • CDw40 antibody
    • GP39 antibody
    • HIGM1 antibody
    • IGM antibody
    • IMD3 antibody
    • MGC9013 antibody
    • Nerve growth factor receptor related B lymphocyte activation molecule antibody
    • OTTHUMP00000031699 antibody
    • OTTHUMP00000031700 antibody
    • p50 antibody
    • T-BAM antibody
    • TBAM antibody
    • TNF receptor superfamily member 5 antibody
    • TNFRSF5 antibody
    • TNR5_HUMAN antibody
    • TRAP antibody
    • Tumor necrosis factor receptor superfamily , member 5 antibody
    • Tumor necrosis factor receptor superfamily member 5 antibody
    • Tumor necrosis factor receptor superfamily member 5 precursor antibody
    • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
    see all

Anti-CD40 antibody 画像

  • IHC image of CD40 staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13545, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-CD40 antibody (ab13545) at 1/1000 dilution

    Lane 1 : Control human HCAEC cells - whole cell lysate
    Lane 2 : Control human HCAEC cells - whole cell lysate
    Lane 3 : Treated human HCAEC cells - whole cell lysate
    Lane 4 : Treated human HCAEC cells - whole cell lysate

    Secondary
    HRP conjugated Goat Anti-Rabbit IgG
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 seconds
    This image is courtesy of an Abreview submitted on 10 August 2005.

    See Abreview

  • All lanes : Anti-CD40 antibody (ab13545) at 1/5000 dilution

    Lane 1 : Human B chronic lymphocytic leukaemia cells, nuclear fraction
    Lane 2 : Human B chronic lymphocytic leukaemia cells, cytoskeletal fraction
    Lane 3 : Human B chronic lymphocytic leukaemia cells, membrane fraction
    Lane 4 : Human B chronic lymphocytic leukaemia cells, cytosolic fraction

    Lysates/proteins at 18 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview

    The primary antibody was incubated with the membrane for 16 hours at 4°C.

    See Abreview


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa


    Exposure time : 8 minutes

Anti-CD40 antibody (ab13545) 使用論文

This product has been referenced in:
  • Shoji T  et al. Local convection-enhanced delivery of an anti-CD40 agonistic monoclonal antibody induces antitumor effects in mouse glioma models. Neuro Oncol 18:1120-8 (2016). Read more (PubMed: 26917236) »
  • Zhang L  et al. In vivo targeted delivery of CD40 shRNA to mouse intestinal dendritic cells by oral administration of recombinant Sacchromyces cerevisiae. Gene Ther 21:709-14 (2014). WB . Read more (PubMed: 24871580) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human tonsil)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Low pH
Permeabilization No
Specification human tonsil
Blocking step Flex endogenouse peroxidase block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative Paraformaldehyde
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投稿 Nov 19 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Low pH
Sample Human Tissue sections (Malignant melanoma/lymphocytes)
Specification Malignant melanoma/lymphocytes
Permeabilization No
Fixative Formaldehyde
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投稿 Jan 20 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (Human B chronic lymphocytic leukaemia cells)
Loading amount 18 µg
Specification Human B chronic lymphocytic leukaemia cells
Gel Running Conditions Reduced Denaturing (12% Tris-Glycine Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Verified customer

投稿 Feb 22 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCAEC)
Specification HCAEC
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Aug 10 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"