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Our Abpromise guarantee covers the use of ab101530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
|IHC-P||Use at an assay dependent concentration.
Antigen retrieval is recommended: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Flow cytometric analysis of rabbit anti-CD4 (SP35) antibody, prediluted, ab101530 in Jurkats cells (green) compared to negative control of rabbit IgG (blue)
ab101530 has not yet been referenced specifically in any publications.