製品の概要

  • 製品名Anti-CD4 antibody [MEM-241] (PerCP)
    CD4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-241] to CD4 (PerCP)
  • 標識PerCP. Ex: 482nm, Em: 675nm
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Human, Cynomolgus Monkey
  • 免疫原

    Two N terminal domains of human CD4 fused to human IgG1 Fc

  • 特記事項

    The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 10µl for 106 cells. ab118658-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • 配列類似性Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻訳後修飾Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • 細胞内局在Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Anti-CD4 antibody [MEM-241] (PerCP) 画像

  • Flow cytometry analysis of Human whole blood after red blod cell lysis, staining CD4 with ab65951. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C.

    Gating Strategy: Live lymphocytes

    See Abreview

  • Flow cytometry analysis of Cynomolgus monkey whole blood after red blod cell lysis, staining CD4 with ab65951. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C.

    Gating Strategy: Live lymphocytes

    See Abreview

Anti-CD4 antibody [MEM-241] (PerCP) (ab65951) 使用論文

This product has been referenced in:
  • Xue L  et al. Prostaglandin D2 activates group 2 innate lymphoid cells through chemoattractant receptor-homologous molecule expressed on TH2 cells. J Allergy Clin Immunol 133:1184-94 (2014). Flow Cyt ; Human . Read more (PubMed: 24388011) »
  • Bastien JP  et al. Photodepletion differentially affects CD4+ Tregs versus CD4+ effector T cells from patients with chronic graft-versus-host disease. Blood 116:4859-69 (2010). Flow Cyt ; Human . Read more (PubMed: 20798236) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: isolated from spleen
Sample buffer: PBS/BSA 1%
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Jan 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: isolated from spleen
Sample buffer: PBS/BSA 1%
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Jan 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Cynomolgus Monkey Cell (from whole blood)
Specification from whole blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Jan 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (from whole blood)
Specification from whole blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Jan 02 2013

Thank you for contacting us. The lab has confirmed that this antibody is indeed specific to CD4. We will guarantee it not to react with any human IgG1 that may be present in your experiments. I hope this helps, please let me know if you need any additi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"