FITC Anti-CD4 抗体 [MEM-241] (ab18281)
Key features and details
- FITC Mouse monoclonal [MEM-241] to CD4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
製品の概要
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製品名
FITC Anti-CD4 antibody [MEM-241]
CD4 一次抗体 製品一覧 -
製品の詳細
FITC Mouse monoclonal [MEM-241] to CD4 -
由来種
Mouse -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Fusion protein (Human).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
MEM-241 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
- APC Anti-CD4 antibody [MEM-241] (ab18280)
- PE Anti-CD4 antibody [MEM-241] (ab18282)
- APC/Cy7® Anti-CD4 antibody [MEM241] (ab233298)
- PE/Cy7® Anti-CD4 antibody [MEM241] (ab233660)
- Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)
- Biotin Anti-CD4 antibody [MEM-241] (ab28069)
- PerCP Anti-CD4 antibody [MEM-241] (ab65951)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18281の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use 4.15µl for 106 cells.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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Flow Cyt
Use 4.15µl for 106 cells. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
配列類似性
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
細胞内局在
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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参照データベース
- Entrez Gene: 920 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- Unigene: 631659 Human
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別名
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
画像
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Overlay histogram showing peripheral blood lymphocytes stained with ab18281 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18281, 0.5 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab18281 は 2 報の論文で使用されています。
- Cui C et al. Propofol maintains Th17/Treg cell balance in elderly patients undergoing lung cancer surgery through GABAA receptor. BMC Immunol 23:58 (2022). PubMed: 36434505
- Eckert AF et al. Measuring ligand-cell surface receptor affinities with axial line-scanning fluorescence correlation spectroscopy. Elife 9:N/A (2020). PubMed: 32441251