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Tissue/ cell preparation (Mouse) - cloned mouse CTL line V4.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.
Our Abpromise guarantee covers the use of ab25505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.
In vivo and in vitro depletion of CD4+ cells; Inhibition of T helper function; Fractionation of CD4+ cells.
|Flow Cyt||Use at an assay dependent concentration.
ab154461-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Flow Cytometry analysis of C57Bl/6 splenocytes labeling CD4 with ab25505 at 0.25µg (solid line). Rat IgG2b isotype control (PE/Cy7®) at 0.25µg (dotted line).
ab25505 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"