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Cloned mouse CTL line V4
In vivo and in vitro treatment with the GK1.5 mAb effectively depletes CD4+ cells. The mAb also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.
Our Abpromise guarantee covers the use of ab41467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent dilution.|
|Flow Cyt||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|Functional Studies||Use at an assay dependent dilution.|
Flow Cytometry analysis of BALB/c mouse splenocyte cells labeling CD4 with ab41467 at 0.1 μg/106 cells dilution. A Rat Anti-Mouse CD8α-FITC was used as the secondary antibody.