Anti-CD4 抗体 [GK1.5] (Biotin) (ab19641)

製品の概要

  • 製品名Anti-CD4 antibody [GK1.5] (Biotin)
    CD4 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [GK1.5] to CD4 (Biotin)
  • 標識Biotin
  • 特異性Recognizes a determinant designated L3T4 present on mouse T-cell surface with an apparent molecular mass of 52kDa. The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
  • アプリケーション適用あり: Flow Cyt, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Lyt-2-negative T-cell clone 243/2.5

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

ab18542 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • 配列類似性Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻訳後修飾Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • 細胞内局在Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Anti-CD4 antibody [GK1.5] (Biotin) 画像

  • C57Bl/6 splenocytes stained with 0.25µg ab19641 (solid line) or 0.25µg biotin-conjugated rat IgG2b isotype control (dashed line), followed by Streptavidin PE.

Anti-CD4 antibody [GK1.5] (Biotin) (ab19641) 使用論文

ab19641 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Tissue sections (Mouse oral tumor cells (MOC-2 obtained from Uppalu)
Specification Mouse oral tumor cells (MOC-2 obtained from Uppalu
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Mar 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"