Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Chicken peripheral blood mononuclear cells were double stained with ab25420 at 0.1 µg/106 cells and a FITC-conjugated mouse anti-chicken CD3 (Clone CT-3) ab24895. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).