翻訳後修飾Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
細胞内局在Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
A typical staining pattern with the B-A1 monoclonal antibody of lymphocytes.
Flow Cytometry - Anti-CD4 antibody [B-A1] (FITC) (ab59474)This image is courtesy of an anonymous Abreview
Flow cytometry analysis of Cynomolgus Monkey whole blood after red blod cell lysis, staining CD4 with ab59474. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C.