FITC Anti-CD4 抗体 [B-A1] (ab59474)
Key features and details
- FITC Mouse monoclonal [B-A1] to CD4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
製品の概要
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製品名
FITC Anti-CD4 antibody [B-A1]
CD4 一次抗体 製品一覧 -
製品の詳細
FITC Mouse monoclonal [B-A1] to CD4 -
由来種
Mouse -
標識
FITC. Ex: 493nm, Em: 528nm -
特異性
Ab59474 recognises a 55 kDa protein.
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アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human CD4. PHA activated T cells
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特記事項
This product was changed from ascites to tissue culture supernatant on 19th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.30
Preservative: 0.09% Sodium azide
Constituents: 94.91% PBS, 5% BSA -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Purified by affinity chromatography. -
ポリ/モノ
モノクローナル -
クローン名
B-A1 -
ミエローマ
x63-Ag8.653 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59474の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt | (4) |
Use at an assay dependent concentration.
ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
特記事項 |
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Flow Cyt
Use at an assay dependent concentration. ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
配列類似性
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
細胞内局在
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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参照データベース
- Entrez Gene: 920 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- Unigene: 631659 Human
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別名
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
画像
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A typical staining pattern with the B-A1 monoclonal antibody of lymphocytes.
This image was generated using the ascites version of the product.
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Flow cytometry analysis of Cynomolgus Monkey whole blood after red blod cell lysis, staining CD4 with ab59474. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C.
Gating Strategy: Live lymphocytesThis image was generated using the ascites version of the product.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab59474 は 7 報の論文で使用されています。
- Yang J et al. Circular RNA CHST15 Sponges miR-155-5p and miR-194-5p to Promote the Immune Escape of Lung Cancer Cells Mediated by PD-L1. Front Oncol 11:595609 (2021). PubMed: 33777742
- Cui C et al. Propofol maintains Th17/Treg cell balance and reduces inflammation in rats with traumatic brain injury via the miR-145-3p/NFATc2/NF-?B axis. Int J Mol Med 48:N/A (2021). PubMed: 34036377
- Meng Q & Qiu B Exosomal MicroRNA-320a Derived From Mesenchymal Stem Cells Regulates Rheumatoid Arthritis Fibroblast-Like Synoviocyte Activation by Suppressing CXCL9 Expression. Front Physiol 11:441 (2020). PubMed: 32528301
- Wu L et al. Mechanisms of M2 Macrophage-Derived Exosomal Long Non-coding RNA PVT1 in Regulating Th17 Cell Response in Experimental Autoimmune Encephalomyelitisa. Front Immunol 11:1934 (2020). PubMed: 33013847
- Di N et al. Effect of combined propofol-sevoflurane anesthesia on immune function in pediatric patients with acute lymphoblastic leukemia. Oncol Lett 18:35-42 (2019). PubMed: 31289469
- Sui M et al. Establishment of specific cytotoxic T lymphocyte culture system and its inhibitory effect on ovarian cancer. Oncol Lett 12:4087-4093 (2016). Flow Cyt ; Human . PubMed: 27895776
- Liu J et al. Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells. Nat Protoc 10:1842-59 (2015). PubMed: 26492140