製品の概要

  • 製品名Anti-CD4 antibody [3-4F4] (FITC)
    CD4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3-4F4] to CD4 (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • 特異性ab24894 recognises CD4.
  • アプリケーション適用あり: Flow Cyt, IP, IHC-Frmore details
  • 種交差性
    交差種: Cat
  • 免疫原

    The details of the immunogen for this antibody are not available.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab106163 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Acetone fixed.

ターゲット情報

  • 機能Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • 配列類似性Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻訳後修飾Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • 細胞内局在Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • 別名
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Anti-CD4 antibody [3-4F4] (FITC) 画像

  • Flow Cytometry analysis of Feline peripheral blood lymphocytes labeling CD4 with ab24894 at 1 μg/106 cells. A Mouse Anti-Feline CD8-PE was used as the secondary antibody.

  • Peripheral blood mononuclear cells from a healthy domestic cat were double-stained with ab24894 (at 1 µg/106 cells) and a PE conjugated mouse anti-feline CD8 antibody (ab25417). Small lymphocytes were then gated and analyzed by two-color flow cytometry on a FACScanTM flow cytometry (BDIS, San Jose, CA).

  • Peripheral blood mononuclear cells from a healthy domestic cat were double-stained with ab24894 and a PE conjugated mouse anti-feline CD5 antibody (ab25415, at 0.2 µg/106 cells). Small lymphocytes were then gated and analyzed by two-color flow cytometry on a FACScanTM flow cytometry (BDIS, San Jose, CA).

Anti-CD4 antibody [3-4F4] (FITC) (ab24894) 使用論文

ab24894 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab24894.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"