製品の概要

  • 製品名
    Anti-CD4 antibody [1F6]
    CD4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1F6] to CD4
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Prokaryotic recombinant protein corresponding to the external domain of the human CD4 molecule.

  • エピトープ
    External domain of the human CD4
  • ポジティブ・コントロール
    • Human skin, mycosis fungoides

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab67001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/20 - 1/40. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Note: The use of H2O2 to block endogenous peroxidase has a detrimental effect on the epitope recognised by ab67001. Solutions containing 1% or greater H2O2 can dramatically REDUCE the level of staining achievable. Blocking after antigen retrieval can also lead to negative results. Therefore, it is recommended that endogenous peroxidase should be blocked with 0.5% H2O2/methanol for 10 minutes BEFORE antigen retrieval. If this recommendation is not followed, staining intensity may be reduced.

ターゲット情報

  • 機能
    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • 配列類似性
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻訳後修飾
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • 細胞内局在
    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

画像

  • CD4 staining of Human skin, mycosis fungoides with 1/20 ab67001. Note membrane staining of infiltrating T lymphocytes. Paraffin section.

プロトコール

参考文献

This product has been referenced in:
  • Ohtani H  et al. Follicular Dendritic Cell Meshwork in Angioimmunoblastic T-Cell Lymphoma Is Characterized by Accumulation of CXCL13(+) Cells. J Clin Exp Hematop 55:61-9 (2015). IHC-P, IF ; Human . Read more (PubMed: 26490517) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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