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Prokaryotic recombinant protein corresponding to the external domain of the human CD4 molecule.
Our Abpromise guarantee covers the use of ab67001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/40. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Note: The use of H2O2 to block endogenous peroxidase has a detrimental effect on the epitope recognised by ab67001. Solutions containing 1% or greater H2O2 can dramatically REDUCE the level of staining achievable. Blocking after antigen retrieval can also lead to negative results. Therefore, it is recommended that endogenous peroxidase should be blocked with 0.5% H2O2/methanol for 10 minutes BEFORE antigen retrieval. If this recommendation is not followed, staining intensity may be reduced.|
ab67001 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"