製品の概要

  • 製品名Anti-CD39 antibody [EPR3678(2)]
    CD39 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3678(2)] to CD39
  • アプリケーション適用あり: WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human CD39.

  • ポジティブ・コントロール
    • Human placenta and IM-9 cell lysates
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
  • 追加情報Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • ターゲット情報

    • 機能In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well.
    • 組織特異性Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only.
    • 配列類似性Belongs to the GDA1/CD39 NTPase family.
    • 翻訳後修飾The N-terminus is blocked.
      Palmitoylated in the N-terminal part.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 製品の状態There are 3 isoforms produced by alternative splicing.
    • 別名
      • ATPDase antibody
      • CD 39 antibody
      • CD39 antibody
      • CD39 antigen antibody
      • DKFZp686D194 antibody
      • DKFZp686I093 antibody
      • Ecto apyrase antibody
      • Ecto ATP diphosphohydrolase antibody
      • Ecto-apyrase antibody
      • Ecto-ATP diphosphohydrolase 1 antibody
      • Ecto-ATPase 1 antibody
      • Ecto-ATPDase 1 antibody
      • Ectonucleoside triphosphate diphosphohydrolase 1 antibody
      • ENTP1_HUMAN antibody
      • ENTPD 1 antibody
      • ENTPD1 antibody
      • FLJ40921 antibody
      • FLJ40959 antibody
      • Lymphoid cell activation antigen antibody
      • NTPDase 1 antibody
      • NTPDase1 antibody
      • SPG64 antibody
      see all

    Anti-CD39 antibody [EPR3678(2)] 画像

    • All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)

      Lane 1 : Rat hippocampus
      Lane 2 : Rat pancreas

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 58 kDa
      Observed band size : 70,74 kDa (why is the actual band size different from the predicted?)

      Blocking and diluting buffer and concentration 5% NFDM/TBST.

    • All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)

      Lane 1 : Mouse placenta
      Lane 2 : Mouse urinary bladder

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 58 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 15 seconds

      Blocking and diluting buffer and concentration 5% NFDM/TBST.

      The PKC beta 2 recombinant protein is GST-tagged, so the molecular weight is higher.

    • Lane 1 : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/100 dilution (purified)
      Lane 2 : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)

      Lane 1 : HUVEC whole cell lysate
      Lane 2 : HUVEC whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 58 kDa
      Observed band size : 70,74 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and diluting buffer and concentration 5% NFDM/TBST.

      Ref: PMID: 18693757

    • All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : IM-9 cell lysate


      Predicted band size : 58 kDa

    Anti-CD39 antibody [EPR3678(2)] (ab108248) 使用論文

    This product has been referenced in:
    • Aldi S  et al. E-NTPDase1/CD39 modulates renin release from heart mast cells during ischemia/reperfusion: a novel cardioprotective role. FASEB J 29:61-9 (2015). Read more (PubMed: 25318477) »
    • Shah D  et al. Extracellular ATP mediates the late phase of neutrophil recruitment to the lung in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol 306:L152-61 (2014). WB . Read more (PubMed: 24285266) »

    See all 4 Publications for this product

    Product Wall

    Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for...

    Read More

    Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    As we discussed on the ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"