Anti-CD39 抗体 [AC2] (ab97552)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [AC2] to CD39
- Suitable for: IHC-Fr, ICC/IF, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD39 antibody [AC2]
CD39 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [AC2] to CD39 -
由来種
Mouse -
アプリケーション
適用あり: IHC-Fr, ICC/IF, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to CD39. EBV-transformed human B lymphoblastoid cell line.
Database link: O75356 -
特記事項
This product was switched from a hybridoma to a recombinant production format on 27th October 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
AC2 -
ミエローマ
x63-Ag8.653 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97552の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
Use a concentration of 2.5 µg/ml.
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ICC/IF |
Use a concentration of 1 µg/ml.
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Flow Cyt |
Use a concentration of 0.2 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IHC-Fr
Use a concentration of 2.5 µg/ml. |
ICC/IF
Use a concentration of 1 µg/ml. |
Flow Cyt
Use a concentration of 0.2 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well. -
組織特異性
Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only. -
配列類似性
Belongs to the GDA1/CD39 NTPase family. -
翻訳後修飾
The N-terminus is blocked.
Palmitoylated in the N-terminal part. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 953 Human
- Omim: 601752 Human
- SwissProt: P49961 Human
- Unigene: 576612 Human
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製品の状態
There are 3 isoforms produced by alternative splicing. -
別名
- ATPDase antibody
- CD 39 antibody
- CD39 antibody
see all
画像
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ab97552 staining CD39 in human peripheral blood mononuclear cells. The cells were fixed with 4% paraformaldehyde (10 min),
permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in
0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab97552 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).Image was acquired with a confocal microscope (LeicaMicrosystems TCS SP8) and a single confocal section is shown.
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Flow cytometry staining of human whole blood with ab97552 (right) or mouse IgG1κ (ab170190) isotype (left). Red blood cells of 200 μl blood were lysed, then cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab97552) or mouse IgG1κ (ab170190) isotype (1x106 in 100 μl at 0.2 μg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice.
The cells were simultaneously stained with CD19 APC.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on alive cells.
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IHC image of CD39 staining in a section of frozen normal human tonsil* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab97552, 1µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
IHC image of CD39 staining in a section of frozen normal human placenta* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab97552, 1µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
IHC image of CD39 staining in a section of frozen normal human pancreas* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab97552, 1µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab97552 は 1 報の論文で使用されています。
- Rowe M et al. Monoclonal antibodies to Epstein-Barr virus-induced, transformation-associated cell surface antigens: binding patterns and effect upon virus-specific T-cell cytotoxicity. Int J Cancer 29:373-81 (1982). PubMed: 6282762