Anti-CD38 抗体 [EPR4106] (ab108403)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4106] to CD38
- Suitable for: WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD38 antibody [EPR4106]
CD38 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4106] to CD38 -
由来種
Rabbit -
特異性
Not suitable for mouse and rat samples because antibody shows a non-specific cytoplasmic staining in almost all tested mouse and rat tissues. -
アプリケーション
適用あり: WB, IHC-P, Flow Cytmore details
適用なし: ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Raji and Ramos cell lysates, Human lymph node lysate. IHC-P: Human endometrial adenocarcinoma, tonsil, lymph node, skeletal muscle and normal colon tissues. Flow Cyt: Raji cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 1.40 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4106 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108403の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).
For unpurified use at 1/1000 - 1/10000. |
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
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Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa). For unpurified use at 1/1000 - 1/10000. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. -
組織特異性
Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. -
配列類似性
Belongs to the ADP-ribosyl cyclase family. -
発生段階
Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 952 Human
- Omim: 107270 Human
- SwissProt: P28907 Human
- Unigene: 479214 Human
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製品の状態
There are 2 isoforms produced by alternative splicing. -
別名
- Acute lymphoblastic leukemia cells antigen CD38 antibody
- ADP ribosyl cyclase 1 antibody
- ADP ribosyl cyclase antibody
see all
画像
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All lanes : Anti-CD38 antibody [EPR4106] (ab108403) at 1/2000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : CD38 knockout A549 cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : HCT 116 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?Anti-CD38 antibody [EPR4106] staining at 1/2000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108403 was shown to bind specifically to CD38. A band was observed at 42 kDa in wild-type A549 cell lysates with no signal observed at this size in CD38 knockout cell line. To generate this image, wild-type and CD38 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Lane 1 : Anti-CD38 antibody [EPR4106] (ab108403) at 1/200 dilution
Lane 2 : Anti-CD38 antibody [EPR4106] (ab108403) at 1/1000 dilution
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte), Whole cell lysate
Lane 2 : Ramos (Human Burkitt's lymphoma B lymphocyte), Whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 34 kDaBuffer and concentration: 5% NFDM/TBST
Exposure time, Lane 1: 20 seconds, Lane 2: 30 seconds
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Anti-CD38 antibody [EPR4106] (ab108403) at 1/3000 dilution + Human lymph node lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 34 kDa
Exposure time: 80 secondsBuffer and concentration: 5% NFDM/TBST
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All lanes : Anti-CD38 antibody [EPR4106] (ab108403) at 1/2000 dilution (purified)
Lane 1 : HuT-78 cell lysate
Lane 2 : Human fetal thymus tissue lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labelling CD38 with ab108403 at 1/900 dilution (1.03 µg/ml) (Red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial adenocarcinoma tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling CD38 with unpurified ab108403.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling CD38 with unpurified ab108403.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Tissue Microarrays stained for "Anti-CD38 antibody [EPR4106]” using "ab108403" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab108403 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab108403 は 16 報の論文で使用されています。
- Chen JW et al. Positive and negative selection shape the human naive B cell repertoire. J Clin Invest 132:N/A (2022). PubMed: 34813502
- Wang X et al. Expanding anti-CD38 immunotherapy for lymphoid malignancies. J Exp Clin Cancer Res 41:210 (2022). PubMed: 35765110
- Moribe F et al. Epigenetic suppression of SLFN11 in germinal center B-cells during B-cell development. PLoS One 16:e0237554 (2021). PubMed: 33513156
- Liu Y et al. A Multi-Center Validated Subtyping Model of Esophageal Cancer Based on Three Metabolism-Related Genes. Front Oncol 11:772145 (2021). PubMed: 34760709
- Zheng XZ et al. Elevation of erythrocyte sedimentation rate and C-reactive protein levels reflects renal interstitial inflammation in drug-induced acute tubulointerstitial nephritis. BMC Nephrol 21:514 (2020). PubMed: 33243164